汉族人群乙型肝炎病毒感染者外周血IL-28B基因多态性研究  

Polymorphisms of peripheral blood IL-28B gene in patients of Han nationality with hepatitis B virus infection

作　　者：谈国蕾[1] 孙梅[1] 王建芳[1] 吴旭平[1] Tan Guolei;Sun Mei;Wang Jianfang;WU Xuping(Institute of Liver Disease Research,Second Affiliated Hospital,Southeast University,Nanjing 210003,Jiangsu Province,China)

机构地区：[1]东南大学附属第二医院肝病研究室,南京市210003

出　　处：《实用肝脏病杂志》2018年第4期561-564,共4页Journal of Practical Hepatology

基　　金：南京市医学科技发展项目(编号:YKK13151/QRX11236)

摘　　要：目的分析乙型肝炎病毒(HBV)感染者外周血IL-28B基因型和等位基因频率分布,并探讨其与疾病病程和进展的关系。方法本研究纳入江苏籍汉族健康人群145例和453例HBV感染者,后者包括无症状HBV携带者(ASC)45例,慢性乙型肝炎患者(CHB)181例,肝硬化(LC)患者69例,肝细胞癌(HCC)患者79例,乙型肝炎肝衰竭(LF)患者79例,采用PCR法和直接测序法检测外周血IL-28B基因rs12979860和rs8099917多态性位点。采用Pearson x2检验对IL-28B rs8099917与rs12979860位点进行Hardy-Weinberg平衡检验,计量资料以(x±s)表示,计数资料采用例数表示。应用SPSS 17.0软件进行方差分析、x2检验和Binary Logistic回归分析。结果 IL-28B基因rs12979860位点有CC、CT和TT 3个基因型,LF患者CC型和C等位基因频率分别为96.2%和98.1%,显著高于健康人群的87.6%和93.1%(OR=0.257,95%CI=0.068~0.973,P=0.045;OR=0.255,95%CI=0.070~0.928,P=0.038);IL-28B基因rs8099917位点有TT、TG和GG 3个基因型,LC患者TT型和T等位基因频率分别为92.8%和96.4%,显著高于健康人群的86.2%和92.4%(OR=0.288,95%CI=0.087~0.948,P=0.041;OR=0.299,95%CI=0.096~0.926,P=0.036)。结论江苏地区汉族人群IL-28B基因多态性与HBV感染后不同疾病表型相关,IL-28B基因rs12979860的C等位基因和IL-28B基因rs8099917的T等位基因可能是HBV感染后病情进展的影响因素。Objective To explore the correlation between blood IL-28B genotype and allele frequencies and clinical phototypes of hepatitis B viral(HBV)infection.Methods A total of 145 healthy persons and 453 patients with HBV infection including 45 with asymptotic HBV carriers(ASC),181 with chronic hepatitis B(CHB),69 with HBV-induced liver cirrhosis(LC),79 with hepatocellular carcinoma(HCC)and 79 with hepatitis B-induced liver failure(LF)in Jiangsu Province were recruited in this study.All the patients and controls were of Han nationality.Peripheral blood IL-28B gene polymorphisms(rs8099917 and rs12979860)were genotyped by PCR and directed sequencing of DNA samples.The IL-28B genotypes and allele frequencies were compared between patients with hepatitis B and healthy controls.Results The genotypes of IL-28B rs12979860 gene showed CC,CT and TT,and LF patients with CC genotype and C allele frequencies were 96.2%and 98.1%,significantly higher than 87.6%and 93.1%,respectively(OR=0.257,95%CI=0.068~0.973,P=0.045;OR=0.255,95%CI=0.070~0.928,P=0.038)in the healthy controls;the genotypes of IL-28B rs8099917 demonstrated TT,TG and GG,and LC patients with TT genotype and T allele frequencies were 92.8%and 96.4%,significantly higher than 86.2%and 92.4%,respectively(OR=0.288,95%CI=0.087~0.948,P=0.041;OR=0.299,95%CI=0.096~0.926,P=0.036)in the healthy controls.Conclusion IL-28B gene polymorphisms might be associated with progression of HBV infection in Han populations in Jiangsu Province.C allele of IL-28B rs12979860 gene and T allele of rs8099917 might be the factors that trigger HBV infection disease deterioration.

关 键 词：乙型肝炎 白细胞介素28B 基因多态性 基因型 等位基因 疾病表型 

分 类 号：R512.62[医药卫生—内科学]