Hsp90AA1识别狂犬病病毒磷蛋白截短体及磷酸化位点突变体的研究  

Study on Ability of Truncated and Phosphorylated Mutants of Rabies Virus Phosphoprotein Recognized by Host Protein Hsp90AA1

作　　者：许运斌[1] 孙玉章 刘娟[3] 颜焰[3] 高兴红[1] 罗果[1] 王欢[1] XU Yun-bin;SUN Yu-zhang;LIU Juan;YAN Yan;GAO Xing-hong;LUO Guo;WANG Huan(Key Laboratory of Infectious&Biosafety,Department of Education Guizhou,Zunyi Medical University,Zunyi,Guizhou,563003,China;Qingdao Vland Biotech Group Co.Ltd,National Research Center for Animal Health Products,Qingdao,Shandong,266000,China;Key Laboratory of Animal Virology of Ministry of Agriculture,Zhejiang University,Hangzhou,Zhejiang,310058,China)

机构地区：[1]遵义医学院贵州省普通高等学校传染病与生物安全特色重点实验室,贵州遵义563000 [2]青岛蔚蓝生物股份有限公司/国家动物用保健品工程技术研究中心,山东青岛266000 [3]浙江大学农业部动物病毒学重点实验室,浙江杭州310058

出　　处：《动物医学进展》2018年第7期32-36,共5页Progress In Veterinary Medicine

基　　金：国家重点研发计划项目(2016YFD0500402);贵州省教育厅资助项目(黔教合KY字[2015]331);贵州省教育厅资助项目(黔教合KY字[2017]185);遵义医学院博士科研启动资金项目(F-812)

摘　　要：为了探究热休克蛋白90AA1(Hsp90AA1)识别狂犬病病毒(RV)磷蛋白(P)截短体及磷酸化位点突变体的能力,构建RV P蛋白多种截短体和磷酸化位点突变体,分别与Hsp90AA1真核表达载体共转染于鼠神经瘤母细胞(N2a)。免疫共沉淀分析显示Hsp90AA1能够广泛识别RV P蛋白的多种截短体和不同的磷酸化突变体。结果提示,Hsp90AA1在RABV感染过程中有着广泛的作用,这对解析RABV复杂的复制机理具有重要的意义。To investigate the ability of truncated and phosphorylated mutants of rabies virus(RV)phosphoprotein(P)recognized by heat shock protein 90AA1(Hsp90AA1),this study constructed a variety of truncated and phosphorylated mutants of RV P protein,which were co-transfected with the eukaryotic expression vector containing the gene Hsp90AA1 into mouse neuroblastoma N2a cells.Immunoprecipitation analysis showed that Hsp90AA1 was able to broadly identify a variety of truncated forms and different phosphorylated mutants of RV P protein.These results suggested that Hsp90AA1 has a broad role in RV infection,which is important for resolving the complex replication mechanism of RV.

关 键 词：Hsp90AA1 狂犬病病毒 磷蛋白 相互作用 

分 类 号：S852.659.5[农业科学—基础兽医学]