酒精对人牙周膜细胞RANKL/OPG基因表达的影响  

Effect of alcohol on mRNA expression of RANKL and OPG in hPDLCs

作　　者：刘贺[1] 钱君荣[1] 马海峰[1] 耿海霞[1] Liu he;Qian Jun-rong;Ma hai-feng;Geng Hai-xia(Department of Stomatology,Affiliated Hospital of Jining Medical University,Shandong Jining 272029,China)

机构地区：[1]济宁医学院附属医院口腔科,山东济宁272029

出　　处：《全科口腔医学电子杂志》2018年第18期10-11,18,共3页Electronic Journal of General Stomatology

基　　金：济宁市科技局项目(济科字[2015]57号-125);济宁医学院科研项目(JY2015KJ028);济宁医学院附属医院"苗圃"科研计划项目(MP-2015-005)

摘　　要：目的研究酒精对人牙周膜细胞RANKL和OPG表达的影响。方法酶消化法结合组织块法分离培养人牙周膜细胞,取4-6代细胞,在0.05 mol/L、0.10 mol/L、0.20 mol/L等不同浓度酒精环境下培养6、12、24、48h,RT-PCR检测RANKL、OPG m RNA的表达。采用SPSS 18.0软件包,对数据进行单因素方差分析。结果 0.05 mol/L、0.10 mol/L、0.20 mol/L浓度实验组RANKL m RNA表达量均高于对照组,具有统计学差异(P<0.05),并且随着酒精浓度的升高,RANKL m RNA表达逐渐升高。0.05 mol/L、0.10 mol/L、0.20mol/L浓度实验组OPG m RNA表达量均低于对照组,具有统计学差异(P<0.05),并且随着酒精浓度的升高,OPG m RNA的表达逐渐下降。结论不同浓度的酒精可以影响人牙周膜细胞中RANKL和OPG m RNA表达,在牙周炎骨吸收中起促进作用。Objective To explore effect of alcohol with different concentration on mRNA expression of RANKL and OPG in hPDLCs.Methods The primary hPDLCs were cultivated with enzyme digestion assay and tissue cultivation.The 4-6 generation of hPDLCs were respectively cultured 6,12,24,48 h in 0.05 mol/L、0.10 mol/L、0.20 mol/L concentration of alcohol.The mRNA expression of RANKL and OPG was detected with RT-PCR.The experimental data was analyzed by one way ANOVA using SPSS 18.0.Result The mRNA expression of RANKL in 0.05 mol/L、0.10 mol/L、0.20 mol/L Concentration alcohol were higher than control group.They increased gradually with the increase of alcohol concentration.The different was significant(P<0.05).The mRNA expression of OPG in 0.05 mol/L、0.10 mol/L、0.20 mol/L concentration alcohol were lower than control group.They decreased gradually with the increase of alcohol concentration.The difference was significant(P<0.05).Conclusion The alcohol with different concentration can regulate the mRNA expression of OPG and and OPG in hPDLCs and will promote bone resorption in periodontitis.

关 键 词：酒精 人牙周膜细胞 κb受体激活物配基 骨保护素 

分 类 号：R781.05[医药卫生—口腔医学]