苦参碱对乳腺癌SK-BR-3细胞增殖、凋亡的影响及机制  

Effects of matrine on proliferation and apoptosis of breast cancer SK-BR-3 cells

作　　者：肖旭[1] 敖曼[1] 石文达[1] 王佳旭 朴宗方 李代晓[1] 胡久丽[1] 王莹[1] 李青山[1] 徐繁[1] XIAO Xu;AO Man;SHI Wenda;WANG Jiaxu;PIAO Zongfang;LI Daixiao;HU Jiuli;WANG Ying;LI Qingshan;XU Fan(The Affiliated Hospital of Chengde Medical College,Chengde 067000,China)

机构地区：[1]承德医学院附属医院,河北承德067000

出　　处：《山东医药》2018年第22期24-27,共4页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题计划(20170886);河北省承德市科学技术研究与发展计划项目(201701A068)

摘　　要：目的探讨苦参碱(MAT)对乳腺癌SK-BR-3细胞增殖、凋亡的影响及相关作用机制。方法取SKBR-3细胞,分别给予不同浓度(0、0.5、1、2、3 mg/L)的MAT干预48 h后,用CCK-8法和流式细胞术检测细胞增殖抑制率和凋亡率,采用实时荧光定量PCR检测细胞miR-21表达,采用Western blotting法检测细胞PTEN蛋白表达。取SK-BR-3细胞,分为mimics-NC+空白培养液组、miR-21 mimics+空白培养液组、mimics-NC+MAT组、miR-21mimics+MAT组,mimics-NC+空白培养液组、mimics-NC+MAT组转染mimics-NC,miR-21 mimics+空白培养液组、miR-21 mimics+MAT组转染miR-21 mimics,且mimics-NC+MAT组、miR-21 mimics+MAT组给予2 mg/L MAT干预,比较各组细胞miR-21、PTEN蛋白表达及凋亡率。结果 0、0.5、1、2、3 mg/L MAT干预乳腺癌SK-BR-3细胞的增殖抑制率分别为0、24.9%±2.8%、33.8%±3.2%、48.9%±5.3%、59.9%±3.1%,凋亡率分别为5.3%±0.3%、19.0%±2.3%、27.2%±2.2%、38.2%±3.0%、46.6%±3.2%,miR-21的相对表达量分别为1.02±0.05、0.75±0.08、0.57±0.06、0.43±0.04、0.28±0.07,PTEN蛋白表达分别为0.14±0.05、0.30±0.06、0.79±0.14、1.11±0.10、1.89±0.22,两两相比P均<0.05。与mimics-NC+空白培养液组相比,miR-21 mimics+空白培养液组miR-21表达高、PTEN蛋白表达低、凋亡率低(P均<0.05),mimics-NC+MAT组miR-21表达低、PTEN蛋白表达高、凋亡率高(P均<0.05);与mimics-NC+MAT组相比,miR-21 mimics+MAT组miR-21表达高、PTEN蛋白表达低、凋亡率低(P均<0.05);与miR-21 mimics+空白培养液组相比,miR-21 mimics+MAT组miR-21表达低、PTEN蛋白表达高、凋亡率高(P均<0.05)。结论 MAT干预能明显抑制乳腺癌细胞SK-BR-3的增殖,诱导其凋亡;这可能是通过抑制miR-21的表达,进而上调PTEN蛋白表达实现的。Objective To explore the effects of matrine(MAT)on the proliferation and apoptosis of breast cancer SK-BR-3 cells as well as the related mechanism.Methods SK-BR-3 cells were treated with MAT at various concentrations(0,0.5,1,2,and 3 mg/L)for 48 h,respectively.Cell proliferation inhibition rate and apoptosis rate were detected by CCK-8 and flow cytometry,respectively.Meanwhile,miR-21 expression and PTEN protein expression in the SK-CR-3 cells were detected by real-time fluorescent quantitative PCR(qPCR)and Western blotting,respectively.The SK-CR-3 cells were divided into mimics-NC+blank culture solution group,miR-21 mimics+blank culture solution group,mimics-NC+MAT group,and miR-21 mimics+MAT group.The cells in the mimics-NC+blank culture solution group and mimics-NC+MAT group were transfected with mimics-NC,the cells in the miR-21 mimics+blank culture solution group and miR-21 mimics+MAT group were transfected with miR-21 mimics,and the cells in the mimics-NC+MAT group and miR-21 mimics+MAT group were given MAT(2 mg/L)for intervention.The miR-21 and PTEN protein expression and apoptosis rate in each group were detected.Results The inhibitory rates of matrine(0,0.5,1,2 and 3 mg/L)on the proliferation of SK-BR-3 cells were 0,24.9%±2.8%,33.8%±3.2%,48.9%±5.3%,and 59.9%±3.1%,respectively;the apoptosis rates were 5.3%±0.3%,19.0%±2.3%,27.2%±2.2%,38.2%±3.0%,and 46.6%±3.2%;the relative expression levels of miR-21 were 1.02±0.05,0.75±0.08,0.57±0.06,0.43±0.04,and 0.28±0.07;the PTEN protein expression levels were 0.14±0.05,0.30±0.06,0.79±0.14,1.11±0.10,and 1.89±0.22,respectively;significant difference was found between these two groups(all P<0.05).Compared with the mimics-NC+blank culture solution group,the miR-21 expression was high,the PTEN protein expression and the apoptosis rate were low in the miR-21 mimics+blank culture solution group(both P<0.05),and the miR-21 expression was low,the PTEN protein expression and the apoptosis rate were high in the mimics-NC+MAT group(all P<0.05).Compared with the m

关 键 词：乳腺癌 苦参碱 微小RNA-21 PTEN蛋白 细胞增殖 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]