一株富含α-亚麻酸栅藻的异养培养条件优化  

OPTIMIZATION OF HETEROTROPHIC GROWTH CONDITIONS FOR A SCENEDESMUS STRAIN WITH HIGH CONTENT OF α-LINOLENIC ACID

作　　者：孟霞 高宏[1] 孔任秋[1] 徐旭东[1] MENG Xia;GAO Hong;KONG Ren-Qiu;XU Xu-Dong(Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2018年第4期819-823,共5页Acta Hydrobiologica Sinica

基　　金：中国科学院科技服务网络计划(KFJ-SW-STS-163)资助~~

摘　　要：HSJ296是本实验室分离纯化的1株能够异养生长、富含α-亚麻酸的栅藻(Scenedesmus sp.)。研究比较了不同温度、氮源和葡萄糖浓度对其生长的影响,结果显示,其最适培养条件为30℃、4 g/L尿素和20—40 g/L葡萄糖。通过分析不同培养条件下HSJ296总脂中的脂肪酸组成,发现主要含有十六碳脂肪酸(C16:0)、油酸(C18:1)、亚油酸(C18:2)和α-亚麻酸(α-C18:3),并且α-亚麻酸的含量稳定在35%—45%。栅藻HSJ296发酵产品或可用作鱼类饲料添加剂以补充α-亚麻酸等营养。HSJ296,a Scenedesmus strain isolated and purified by this lab,can grow heterotrophically in the dark and contains a relatively high amount ofα-linolenic acid.Comparisons of heterotrophic growth at different temperatures and concentrations of glucose,or with different nitrogen sources,indicated that the optimal culture conditions included 30℃,4 g/L urea and 20-40 g/L glucose.As shown with fatty acid composition analyses under different conditions,the fatty acids of HSJ296 were mainly composed of hexadecanoic acid(C16:0),oleic acid(C18:1),linoleic acid(C18:2)andα-linolenic acid(α-C18:3),and theα-linolenic acid content in total lipids was between 35%-45%in independent experiments.The fermentation product of Scenedesmus HSJ296 may be used as a fish dietary supplement to supplyα-linolenic acid and other nutrients.

关 键 词：栅藻HSJ296 Α-亚麻酸 异养生长 

分 类 号：S917.3[农业科学—水产科学]