油茶枯中两个多酚化合物的提取分离及其抗炎活性研究  

Extraction and Anti-inflammatory Activities of Two Polyphenols from the Cake of Camellia oleifera Abel.

作　　者：黎国庆 许承婷[1,2] 王立欣 黄俊 焦兵 覃江克[1,2] LI Guoqing;XU Chengting;WANG Lixin;HUANG Jun;JIAO Bing;QIN Jiangke(College of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin Guangxi 541004,China;State Key Laboratory for Chemistry and Molecular Engenineering of Medicinal Resources,Guangxi Normal University, Guilin Guangxi 541004,China;Department of Food and Biotechnology,Guangxi Vocational and Technical College, Nanning Guangxi 530226,China)

机构地区：[1]广西师范大学化学与药学学院,广西桂林541004 [2]广西师范大学省部共建药用资源化学与药物分子工程国家重点实验室,广西桂林541004 [3]广西职业技术学院食品与生物技术系,广西南宁530226

出　　处：《广西师范大学学报（自然科学版）》2018年第3期68-75,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(21462007);广西科学研究与技术开发计划(桂科攻1598007-19);省部共建药用资源化学与药物分子工程国家重点实验室支持项目(EMEMR 2016-A08;2015-A03)

摘　　要：为深入研究油茶中的多酚化合物及其药理活性,以油茶枯为原料,经石油醚脱脂、乙醇提取、2次中低压制备色谱制备得到2个多酚化合物山奈酚-3-O-[2-O-β-D-半乳糖-6-O-α-L-鼠李糖]-β-D-葡萄糖苷(1)和山奈酚-3-O-[2-O-β-D-木糖-6-O-α-L-鼠李糖]-β-D-葡萄糖(2),并采用MTT法检测多酚化合物对巨噬细胞RAW264.7的毒性作用;利用脂多糖LPS刺激巨噬细胞RAW264.7建立炎症模型,采用ELISA试剂盒测定油茶多酚1,2及混合物3的抗炎活性。结果表明,多酚化合物1、2及其混合物3对小鼠巨噬细胞RAW264.7毒性不大,且均能抑制TNF-α、NO、i NOS的表达释放,表现出良好的抗炎活性,在药物研究与开发上具有良好发展前景。For providing the theoretical foundation and experiment evidence to further study on the polyphenols from the cake of Camellia oleifera Abel.and their pharmacological activities,two polyphenols compounds(kaempferol-3-O-[2-O-β-D-galactopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranosie(1)and kaempferol-3-O-[2-O-β-D-xylopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside(2))from the cake of C.oleifera Abel.were obtained through degrease,ethanol extraction and prep-MPLC.MTT method was used to evaluate the cytotoxicity on RAW264.7 cell,establish cell model of LPS-induced inflammation and detect the anti-inflammatory activities of flavone glycosides compound 1,2 and the mixture 3.The results of anti-inflammatory activity assay showed that the flavone glycosides compound 1,2 and the mixture 3 were less toxic to RAW264.7 and both of them could significantly suppress the production of NO,i NOS,TNF-αin cells,which indicated that both of them had good anti-inflammatory effects and good prospect in drug research and development.

关 键 词：油茶枯 多酚 提取 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]