β连环蛋白对脑胶质瘤辐射抗性的影响  

Effect of β-catenin on radiation resistance of glioma

作　　者：高力扬[1] 李锦宏 陈兵 杨帆 岑学程 廖壮槟 龙霄翱 王思捷 Li-yang Gao;Jin-hong Li;Bing Chen;Fan Yang;Xue-cheng Cen;Zhuang-bin Liao;Xiao-ao Long;Si-jie Wang(School of Life Science,Ningxia University,Yinchuan,Ningxia 750021,China;Department of Neurosurgery,Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001,China;Clinical Research Center,Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001,China)

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]广东医科大学附属医院神经外科,广东湛江524001 [3]广东医科大学附属医院临床研究中心,广东湛江524001

出　　处：《中国现代医学杂志》2018年第20期28-33,共6页China Journal of Modern Medicine

摘　　要：目的探讨β连环蛋白(β-catenin)在X射线照射条件下对人脑胶质瘤U87细胞增殖、生存的影响。方法 U87细胞经采用5μmol/L IWR-1-endo处理后,用X射线照射,采用CCK-8法平板克隆形成实验检验细胞增殖和存活能力,免疫荧光染色检验细胞相关蛋白的表达,电镜观察细胞内微结构的变化。结果人脑胶质瘤U87细胞辐射后,照射组β-catenin蛋白相对表达量比对照组低(P<0.05);经IWR-1-endo处理的细胞增殖和平板克隆形成率有增加趋势(P<0.05)。结论降低β-catenin抑制Wnt/β-catenin信号通路,虽然能降低胶质瘤细胞增殖能力,但可能通过保护细胞线粒体的方式增加胶质瘤细胞对辐射治疗的抗性。Objective To explore the effect ofβ-catenin on proliferation and survive of U87 glioma cells after radiation.Methods To reduce the accumulation ofβ-catenin,U87 glioma cells were treated with 5μmol/L IWR-1-endo.CCK-8 and single colony formation assay were used to examine the proliferation and survival of U87 cells after radiation.And immunocytochemistry(ICC)was used to monitor the expressions of related proteins in the cells.Moreover,electron microscopy was used to observe the changes of microstructures of the cells.Results The expression ofβ-catenin decreased dramatically in the U87 cells after radiation treatment compared to the control group(P<0.05).After radiation,the proliferation of the IWR-1-endo-treated cells and the plate colony-forming efficiency increased(P<0.05).Conclusions Decreased accumulation ofβ-catenin might reduce the proliferation of glioma cells through inhibition of the Wnt/β-catenin signaling pathway,but it might cause radiation resistance of glioma cells by protecting the mitochondria of the cells.

关 键 词：人脑胶质瘤 Β-CATENIN 辐射抗性 

分 类 号：R739.41[医药卫生—肿瘤]