饲粮中添加表皮生长因子对断奶仔猪血清生化指标、血清游离氨基酸和小肠黏膜水解氨基酸含量的影响  

Effects of Dietary Epidermal Growth Factor on Serum Biochemical Indices,Serum Free Amino Acid and Intestinal Mucosal Hydrolytic Amino Acid Contents of Weaned Piglets

作　　者：朱繁 王丽霞 贾杏林[1] 尹佳 杨焕胜[2,3] 印遇龙 ZHU Fan;WANG Lixia;JIA Xinglin;YIN Jia;YANG Huansheng;YIN Yulong(Animal Medical School of Hunan Agricultural University,Changsha 410128,China;Key Laboratory of Subtropical Agricultural Ecological Process of Chinese Academy of Sciences,Sub-Tropical Agricultural Ecology Research Institute of Chinese Academy of Sciences,Changsha 410125,China;Animal Nutrition And Human Health Laboratory,Life Sciences College,Hunan Normal University,Changsha 410081,China)

机构地区：[1]湖南农业大学动物医学院,长沙410128 [2]中国科学院亚热带农业生态研究所,中国科学院亚热带农业生态过程重点实验室,长沙410125 [3]湖南师范大学生命科学学院,动物营养与人体健康实验室,长沙410081

出　　处：《动物营养学报》2018年第7期2519-2528,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31402089,31330075)。

摘　　要：本试验旨在研究饲粮中添加表皮生长因子(EGF)对断奶仔猪血清生化指标、血清游离氨基酸和小肠黏膜水解氨基酸含量的影响。选取体重相近、健康的21日龄断奶仔猪42头,随机分成3组,每组14个重复,每个重复1头仔猪。Ⅰ组为对照组,饲喂基础饲粮;Ⅱ组和Ⅲ组分别饲喂在基础饲粮中添加200和400μg/kg EGF的试验饲粮。试验期为14 d。结果表明:1)饲粮中添加EGF对断奶仔猪平均日增重、平均日采食量和料重比没有显著影响(P>0.05)。2)断奶后7 d,Ⅱ组血清尿酸含量显著高于Ⅰ组(P<0.05),而Ⅱ组和Ⅲ组血清免疫球蛋白A含量显著低于Ⅰ组(P<0.05)。断奶后14 d,Ⅲ组血清肌酐含量显著低于Ⅰ组(P<0.05),且Ⅲ组血清谷草转氨酶/谷丙转氨酶值显著低于Ⅰ组和Ⅱ组(P<0.05)。饲粮中添加EGF对断奶仔猪血清尿素氮、葡萄糖、总蛋白、白蛋白、球蛋白、免疫球蛋白M、免疫球蛋白G、胰岛素样生长因子-1、生长激素含量,白蛋白/球蛋白,谷草转氨酶、谷丙转氨酶活性没有显著影响(P>0.05)。3)断奶后7 d,Ⅱ组血清牛磺酸、丙氨酸、瓜氨酸和组氨酸含量显著低于Ⅲ组(P<0.05),Ⅱ组血清精氨酸、谷氨酸和丝氨酸含量显著低于Ⅰ组和Ⅲ组(P<0.05),各组之间其他血清氨基酸含量无显著差异(P>0.05)。4)断奶后14 d,与Ⅰ组和Ⅲ组比,Ⅱ组空肠黏膜缬氨酸和回肠黏膜半胱氨酸含量显著升高(P<0.05),Ⅱ组空肠黏膜丝氨酸、谷氨酸、丙氨酸、半胱氨酸、异亮氨酸、苯丙氨酸和组氨酸含量也有升高趋势(P<0.10)。综上所述,饲粮中添加高剂量EGF能够降低断奶仔猪血清免疫球蛋白A等生化指标含量,而饲粮中添加低剂量EGF能够降低血清和小肠黏膜部分氨基酸含量。The objective of this experiment was to investigate the effects of dietary epidermal growth factor(EGF)on serum biochemical index,serum free amino acid and intestinal mucosal hydrolytic amino acid contents of weaned piglets.Forty-two healthy piglets with similar body weight were weaned at 21 days of age and randomly assigned into 3 groups with 14 replicates per group and 1 piglet per replicate.Piglets in groupⅠ(control group)were fed a basal diet,and the others in groupsⅡandⅢwere fed the basal diet supplemented with 200 and 400μg/kg EGF,respectively.The experiment lasted for 14 days.The results showed as follows:1)dietary supplemented with EGF had no significant effects on average daily gain,average daily feed intake and feed to gain ratio of weaned piglets(P>0.05).2)After weaning 7 days,the serum uric acid content in groupⅡwas significantly higher than that in groupⅠ(P<0.05),the serum immunoglobulin A content in groupsⅡandⅢwas significantly lower than that in groupⅠ(P<0.05).After weaning 14 days,the serum creatinine content in groupⅢwas significantly lower than that in groupⅠ(P<0.05),the serum glutamic-oxalacetic transaminase/glutamic-pyruvic transaminase value in groupⅢwas significantly lower than that in groupsⅠandⅡ(P<0.05).Dietary supplemented with EGF had no significant effects on serum urea nitrogen,glucose,total protein,albumin,globulin,immunoglobulin M,immunoglobulin G,insulin-like growth factor-1,growth hormone contents,and albumin/globulin and glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase activities of weaned piglets(P>0.05).3)After weaning 7 days,the contents of taurine,alanine,citrullinine and histidine in serum in groupⅡwere significantly lower than those in groupⅢ(P<0.05),the contents of arginine,glutamate and serine in serum in groupⅡwere significantly lower than those in groupsⅠandⅢ(P<0.05),and there were no significant differences on other serum amino acid contents among all groups(P>0.05).4)After weaning 14 days,compared with groupsⅠand

关 键 词：EGF 断奶仔猪 血清生化指标 氨基酸 肠道 

分 类 号：S828[农业科学—畜牧学]