FK506对肝脏保存再灌注中Bcl-2mRNA、Bax mRNA表达及肝细胞凋亡的影响  

The effects of FK506 on expression of Bcl-2 mRNA,Bax mRNA and hepatocyte apoptosis in hepatic preservation and reperfusion

作　　者：赵浩亮[1] 韩振国[1] 李正中[1] 

机构地区：[1]山西医科大学第一医院普外科,太原030001

出　　处：《中华实验外科杂志》2002年第6期535-537,共3页Chinese Journal of Experimental Surgery

基　　金：山西省自然科学基金资助项目 ( 199910 95)

摘　　要：目的　探讨肝脏保存再灌注中Bcl 2mRNA、BaxmRNA表达 ,肝细胞凋亡以及FK50 6的作用。方法　建立离体大鼠肝脏保存再灌注模型 ,采用逆转录 多聚酶链反应 (RT PCR)和细胞凋亡原位末端标记技术 (TUNEL) ,分别检测肝脏保存 0、8、1 6、2 4、32h组Bcl 2mRNA、BaxmRNA表达 ,肝细胞凋亡以及FK50 6对上述指标的影响。结果　FK50 6保存组 1 6、2 4、32hBcl 2mRNA表达明显高于对照组 (P <0 .0 5) ;BaxmRNA表达明显低于对照组 (P <0 .0 5) ;FK50 6保存组肝细胞凋亡指数明显低于对照组 (P <0 .0 5)。结论　FK50 6能使肝脏保存再灌注中Bcl 2mRNA表达增强 ,BaxmRNA表达减低 ,肝细胞凋亡减少。FK50 6对肝脏保存再灌注损伤具有防治作用。Objective To investigate Bcl 2 mRNA,BaxmRNA expression and hepatocyte apoptosis in hepatic preservation and reperfusion and effect of FK506.Methods The model of the preservation reperfusion of isolated rat liver was established.Liver tissue Bcl 2 mRNA,Bax mRNA expression and hepatocyte apoptosis were studied respectively with reverse transcription polymerase chain reaction (RT PCR) and in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique at different preservation reperfusion time points (0,8,16,24,32?h).Results In FK506 treated group, the Bcl 2 mRNA expression was obviously increased (P<0.05).Bax mRNA expression was significantly decreased (P<0.05).Hepatocyte apoptosis index was remarkably lowered (P<0.05), compared correspendingly with control group after preservation for 16,24 and 32?h followed by the same reperfusion time.Conclusion FK506 can increase Bcl 2 mRNA expression,decrease Bax mRNA expression and inhibit hepatocyte apoptosis.FK506 may paly a positive role in preventing hepatic preservation reperfusion injury.

关 键 词：FK506 肝脏保存 再灌注 Bcl-2 MRNA Bax MRNA 肝细胞凋亡 

分 类 号：R363[医药卫生—病理学]