转基因动物模型制作进展  

Development of Transgenic Animal Models

作　　者：孙甫[1] 杨姣 吴文焕 王磊[1] 田宇[1] SUN Fu;YANG Jiao;WU Wen-huan;WANG Lei;TIAN Yu(First Affiliated Hospital of Xi'an Medical College,Xi'an 710077,Shaanxi,China;Xi'an Medical College,Xi'an 710021,Shaanxi,China)

机构地区：[1]西安医学院第一附属医院,陕西西安710077 [2]西安医学院,陕西西安710021

出　　处：《医学信息》2018年第1期35-37,共3页Journal of Medical Information

基　　金：国家自然科学基金:凋亡基因﹑应力及其交互作用与腰椎间盘突出症的关联及个体化预警体系建立(编号:81171761);陕西省自然科学基金:新型纳米分子细胞递送技术在椎间盘退变干预过程中的作用(编号:2017SF-088)

摘　　要：转基因动物是应用实验的方法将外源基因导入到早期的胚胎内,使之可以在动物染色体基因组内稳定的整合,并能遗传给后代的一类动物。随着转基因技术以及分子遗传学的发展,转基因动物模型逐渐应用在医学科学的各个领域。本文对转基因动物模型的几种常用建模方法做一综述,探讨各种不同的转基因动物模型制作方法的优劣。最后以显微注射法为例简述制作转基因动物的方法。Transgenic animals are the experimental methods used to introduce exogenous genes into early embryos so that they can be stably integrated in the genome of animal genomes and can be passed on to offspring animals.With the development of transgenic technology and molecular genetics,transgenic animal models are gradually applied in various fields of medical science.This article reviews several commonly used modeling methods for transgenic animal models and discusses the advantages and disadvantages of various methods for making transgenic animal models.Finally,the microinjection method as an example to describe the method of making transgenic animals.

关 键 词：转基因 动物模型 显微注射法 

分 类 号：R-332[医药卫生]