平菇菌丝液体培养基的优化  

Optimization of Liquid Medium for Mycelium Culture of Pleurotus ostreatus

作　　者：曹秀敏 胡巍 张子豪 刘颖 陈银银 石玉 CAO Xiu-min;HU Wei;ZHANG Zi-hao(College of Food and Biotechnology,Wuhan Institute of Design and Sciences,Wuhan,Hubei 430205)

机构地区：[1]武汉设计工程学院食品与生物科技学院,湖北武汉430205

出　　处：《安徽农业科学》2018年第21期15-17,共3页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生科技创新项目(201614035001)

摘　　要：[目的]研究平菇菌丝液体培养基的最佳配方。[方法]以平菇为试材,以菌丝干重为检测指标,筛选液体发酵培养基的最佳碳源和氮源,通过单因素试验和正交试验,研究供试菌株液体发酵的最佳培养基配方。[结果]供试菌株液体发酵的最佳培养基配方:马铃薯淀粉4%、酵母粉0.100%、硫酸镁0.15%、磷酸氢二钾0.2%。[结论]供试菌株在优化培养基配方条件下,菌丝干重有显著提高,为平菇液体菌种的培养和规模化栽培提供了理论依据。[Objective]To study the optimal formula of liquid medium for mycelium culture of Pleurotus ostreatus.[Method]Taking P.ostreatus as experimental materials,mycelial dry wet was selected for detection index,the optimum carbon source and nitrogen source has been chosen with fermentation medium to maximize mycelia growth.The optimal test strain liquid fermentation medium formula was determined by univariate test and orthogonal test.[Result]The optimal formula was 4%of potato flour,0.100%of yeast extract,0.15%of magnesium sulfate and 0.2%of dipotassium hydrogen phosphate.[Conclusion]The mycelium biomass of the tested strain was increased significantly under the condition of the optimal formula,which provided a theoretical basis for the cultivation of liquid strains and large-scale cultivation of P.ostreatus.

关 键 词：平菇 液体培养基 培养条件优化 菌丝干重 

分 类 号：S646.14[农业科学—蔬菜学]