山东省苍山大蒜(Allium sativum)贮藏期新病原菌的分离与鉴定  

Identification and Isolation of New Pathogenic Fungi of Cangshan Garlic Storage Diseases from Shandong Province

作　　者：钟文文 刘婷婷 赵志龙 高翠娟[2] 安冬玉 王一宇 许俊杰 ZHONG Wen-wen;LIU Ting-ting;ZHAO Zhi-long(College of Pharmacy,Linyi University,Linyi,Shandong 276000)

机构地区：[1]临沂大学药学院,山东临沂276000 [2]临沂大学生命科学学院,山东临沂276000

出　　处：《安徽农业科学》2018年第21期140-144,151,共6页Journal of Anhui Agricultural Sciences

基　　金：2017年山东省自然基金项目(联合专项)(ZR2017LC001);2017年临沂大学大学生创新创业训练项目校级重点项目(201710452024)

摘　　要：对临沂市兰陵县苍山大蒜贮藏期病害情况进行了调查,采用柯赫氏法则和病斑显微观察法对病原菌株lyu20170012进行了验证,并通过形态学特征和r DNA ITS序列分析对其进行了鉴定。结果表明,从病斑上分离得到4种真菌和1种细菌,经致病性检测发现菌株lyu20170012具有致病性,将lyu20170012菌株鉴定为尖孢镰刀菌(Fusarium oxysporum)。The investigation on Cangshan garlic storage diseases in Lanling County of Linyi City was carried out.The pathogenity of pathogenic strain lyu20170012 was detected and verified by the Koch and the microscopic observation.Results showed as followed:four kinds of fungi and one kind of bacteria were isolated from the Cangshan garlic lesion;the lyu20170012 strain was identified as Fusarium oxysporum by morphological characteristics and rDNA ITS sequence analysis.

关 键 词：尖孢镰刀菌 大蒜鳞茎病害 柯赫氏法则 病原菌 

分 类 号：S432.42[农业科学—植物病理学]