柱后基体分离-高效液相色谱质谱法测定烟草中香味成分  

Determination of Aroma Components in Tobacco by HPLC-MS/MS Based on Post-Colum Matrix Separation Technique

作　　者：田振锋 马丽伊 孔俊[1] 林作敏 高芸[2] 淦五二[2] TIAN Zhen-feng;MA Li-yi;KONG Jun(Anhui Tobacco Industrial Co.,Ltd.Technology Center,Hefei,Anhui 230088;Department of Chemistry,University of Science and Technology of China,Hefei,Anhui 230026)

机构地区：[1]安徽中烟工业有限责任公司技术中心,安徽合肥230088 [2]中国科学技术大学化学系,安徽合肥230026

出　　处：《安徽农业科学》2018年第21期178-181,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽中烟工业有限责任公司联合实验室科技项目(2015124)

摘　　要：[目的]提取烟草中的香味成分,并用LC-MS/MS方法对其进行测定和分析。[方法]烟草样品经甲醇溶液超声萃取后,取上清液直接进样LC-MS/MS分析,采用电喷雾正离子多反应监测方式检测,并采用柱后预分离糖基体,消除其对香味成分离子化的影响。[结果]各香味成分线性关系良好(R^2≥0.982 2),加标回收率为82.43%~95.77%,平均相对标准偏差(RSD)为2.00%~4.58%。[结论]该方法灵敏、准确,分析速度快,适合烟草中香味成分检测。[Objective]Aroma components in tobacco were extracted and assayed and analyzed by LC-MS/MS method.[Method]After the tobacco sample was extracted by the methanol solution,the supernatant was directly analyzed by LC-MS/MS.Electrospray positive ion multiple reaction monitoring was used to detect and use the post-column pre-separation of the sugar matrix to eliminate its effect on the ionization of aroma components.[Result]The linear relationship of the flavor components was good(R2≥0.982 2),the spike recovery was 82.43%-95.77%,and the average relative standard deviation(RSD)was 2.00%-4.58%.[Conclusion]The method is sensitive,accurate and analysis speed fast,which is suitable for the detection of aroma components in tobacco.

关 键 词：烟草 香味成分 LC-MS/MS 超声提取 

分 类 号：TS424[农业科学—烟草工业]