虾青素对脂多糖通过TLR4/MyD88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响  

Effects of astaxanthin on IPEC-J2 cell inflammation induced by lipopolysaccharide via TLR4/MyD88/NF-κB signaling pathway

作　　者：朱凌羽 张子琪 兰海楠[1] 吴旻 刘惠麟 郑鑫[1] ZHU Lingyu;ZHANG Ziqi;LAN Hainan;WU Min;LIU Huilin;ZHENG Xin(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Chinese Medicinal College,Jilin Agricultural University,Changchun 130118,China;College of Life Sciences,Jilin University,Changchun 130012,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118 [3]吉林大学生命科学学院,吉林长春130012

出　　处：《华南农业大学学报》2018年第5期53-58,共6页Journal of South China Agricultural University

基　　金：国家自然科学基金(31672511)

摘　　要：【目的】研究虾青素(AST)对脂多糖(LPS)通过TLR4/My D88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响。【方法】采用MTT法确定虾青素和LPS对IPEC-J2细胞和转染IPEC-J2细胞的最佳处理浓度和处理时间,在处理后采用实时荧光定量PCR检测IPEC-J2细胞和转染IPEC-J2细胞炎症因子NF-κB、TNF-α、IL-6和IL^(-1)β的m RNA相对表达量,采用ELISA检测上述炎症因子的分泌量。【结果】当处理3 h,虾青素浓度达到150μmol·L^(-1)时,IPEC-J2细胞和转染IPEC-J2细胞活力达到峰值;当处理6 h,LPS质量浓度达到100 ng·m L^(-1)时,IPEC-J2细胞和转染IPEC-J2细胞活力达到峰值。与对照组相比,LPS组IPEC-J2细胞NF-κB、TNF-α、IL-6、IL^(-1)β的m RNA相对表达量和分泌量显著升高(P<0.05);与LPS组相比,AST+LPS组NF-κB、TNF-α、IL-6、IL^(-1)β在IPEC-J2细胞中的m RNA相对表达量和分泌量显著降低(P<0.05),而在转染IPEC-J2细胞中的炎症因子m RNA相对表达量和分泌量均无显著变化(P>0.05)。【结论】虾青素可以抑制细胞炎症反应,且对细胞的保护作用与TLR4/My D88/NF-κB信号通路相关。【Objective】To investigate the effect of astaxanthin(AST)on IPEC-J2 cell inflammation induced by lipopolysaccharide(LPS)via TLR4/MyD88/NF-κB signaling pathway.【Method】MTT assay was performed to determine the optimal time and concentrations of astaxanthin and LPS for treating IPEC-J2 cells and transfected IPEC-J2 cells.Fluorescent quantitative RT-PCR was performed to determine the relative mRNA expressions of inflammatory factors including NF-κB,TNF-α,IL-6 and IL-1βin IPEC-J2 cells and transfected IPEC-J2 cells stimulated by LPS.ELISA assays were carried out to determine the secretion amounts of these inflammatory factors.【Result】The vitality of IPEC-J2 cells and transfected IPEC-J2 cells reached the peak when treated with 150μmol·L^–1 astaxanthin for 3 h or 100 ng·mL^–1 lipopolysaccharide for 6 h.Compared with control group,the relative mRNA expressions and secretions of NF-κB,TNF-α,IL-6 and IL-1βin IPEC-J2 cells significantly increased in LPS treatment group(P<0.05).Compared with LPS treatment group,the relative mRNA expressions and secretions of NF-κB,TNF-α,IL-6 and IL-1βin LPS+AST group were significantly lower in IPEC-J2 cells(P<0.05),but did not differ significantly in transfected IPEC-J2 cells(P>0.05).【Conclusion】Astaxanthin can inhibit cell inflammation,and its protective effect on cells is related to TLR4/MyD88/NF-κB signaling pathway.

关 键 词：虾青素 脂多糖 IPEC-J2细胞 转染IPEC-J2细胞 TLR4/MyD88/NF-κB信号通路 炎症因子 

分 类 号：S813[农业科学—畜牧学]