妊娠小鼠子宫内胎鼠腹腔注射人肿瘤HepG2细胞对胎鼠生长发育的影响  

Effect of in utero intraperitoneal injection of human tumor HepG2 cells on the growth and development of fetal mice

作　　者：李姗姗[1] 舒昀 陈来[1] 李俊[1] 陈丽玲[1] 曾文婷 袁可望 舒坤[1] 徐彭[1] 罗小泉[1] LI Shanshan;SHU Yun;CHEN Lai;LI Jun;CHEN Liling;ZENG Wenting;YUAN Kewang;SHU Kun;XU Peng;LUO Xiaoquan(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学,南昌330004

出　　处：《中国比较医学杂志》2018年第7期33-42,共10页Chinese Journal of Comparative Medicine

基　　金：江西省青年科学基金(编号:20161BAB215214);江西省教育厅科学技术研究项目基金(编号:GJJ14623)

摘　　要：目的探讨妊娠小鼠子宫内胎鼠腹腔注射人肿瘤HepG2细胞对胎鼠生长发育的影响。方法将孕鼠随机分为5组,细胞注射组在孕14 d胎鼠腹腔注射不同浓度的HepG2细胞悬液,并设假手术组及对照组,记录孕鼠生产情况、出生小鼠的体重,并对子代鼠的发育学指标进行评价,选取成年后小鼠解剖观察肝、肺、肾等主要脏器有无畸形、病变,并取材进行HE染色,显微观察各组小鼠主要脏器的病理改变情况。结果与对照组相比,子宫内胎鼠腹腔各细胞注射组子代鼠的体重和生长发育指标差异无显著性,肉眼观察各主要脏器无病变,显微观察未见异常结构。结论子宫内胎鼠腹腔注射人肿瘤HepG2细胞不会对胎鼠发育产生影响。Objective To investigate the effect of in utero intraperitoneal injection of human tumor HepG2 cells on the growth and development of fetal mice.Methods Pregnant mice were randomly divided into five groups and various densities of HepG2 cell suspensions were injected into the fetus by intrauterine injection at embryonic day 14.Sham operation and control groups were included.Pregnancies and the body weight of offspring were recorded,and developmental indicators were evaluated.Adult mice were selected and dissected to observe malformations and pathological changes in major organs including the liver,lungs,and kidneys.Results There was no significant difference in the growth or development of offspring in HepG2 cell injection and control groups.There were no lesions in major organs based on macroscopic and microstructural observations.Conclusions In utero intraperitoneal injection of human tumor HepG2 cells has no effect on the development of fetal mice.

关 键 词：子宫内胎鼠腹腔注射 获得性免疫耐受 肿瘤动物模型 

分 类 号：R-33[医药卫生]