狨猴人工辅助生殖技术及基因修饰模型研究进展  

Review of marmoset assisted reproductive technology and their gene-modified models

作　　者：滕永康[1] 刘云波[1] TENG Yongkang;LIU Yunbo(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS);Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2018年第7期107-112,共6页Chinese Journal of Comparative Medicine

基　　金：北京市科技计划(编号:Z161100000216154);国家重点研究计划(编号:2017YFA0105201)。

摘　　要：普通绵耳狨猴是体型较小的非人灵长类实验动物,具有饲养成本低,繁殖效率高,性成熟时间短等优势,非常适合开展辅助生殖技术及基因修饰模型研究。狨猴的人工辅助生殖技术(assisted reproduction technology,ART)主要包括:精液采集、超数排卵、卵母细胞采集、体外成熟、体外授精、胚胎培养和移植等。目前各项技术的研究较全面,但仍有细节亟需完善。随着基因编辑技术的兴起,基于人工辅助生殖技术的狨猴基因修饰研究逐渐成为热点。2009年日本首次成功开展转基因狨猴研究,截至目前,中国尚无基因修饰狨猴相关报道。本文就近年来狨猴人工辅助生殖技术,以及狨猴基因修饰研究进展进行综述。Marmosets,a kind of small non-human primates,are very suitable for research by gene modification because of their lower feeding cost and higher efficiency,shorter time of sexual maturation than macaques.Any model established by gene modification cannot be produced without assisted reproduction technology(ART)consisting of semen collection,superovulation,oocyte retrieval,in vitro maturation,in vitro fertilization,and embryo transplantation.At present,the research of these techniques is relatively comprehensive,but there are still details that need improvement.Recently,studies of gene-modified marmosets based on ART have gradually become a research focus with the development of gene editing technology.In 2009,the first report of transgenic marmosets was published by Japanese scientists,whereas related reports have not been published by Chinese scientists.This article reviews the recent progress of marmoset ART and their gene-modified models.

关 键 词：狨猴 辅助生殖 基因修饰 

分 类 号：R-33[医药卫生]