芳香族致敏与HLA-B~*1502基因多态性之间的相关性研究  

A study on the correlation between aromatic sensitization and HLA-B~*1502 gene polymorphism

作　　者：侯蕴祈 石海琴 莫石兰 杨红姬 游文霞 邓颖欣 

机构地区：[1]南方医科大学顺德医院/佛山市顺德区第一人民医院,广东佛山528300

出　　处：《中医临床研究》2018年第15期88-90,共3页Clinical Journal Of Chinese Medicine

基　　金：2015佛山市科技计划项目;项目编号:2015AB002023

摘　　要：目的:遗传医学和预防医学的发展开辟了新的领域,芳香族致敏在身体经酶代谢后与蛋白多肽形成半抗原复合物,可诱发后来的过敏反应,本研究分析HLA-B*1502基因多态性与芳香族致敏发病风险及治疗疗效的关系。方法:选取43例芳香族致敏患者作为试验组,另选取110例同期体检健康者作为对照组,分析HLA-B*1502基因的多态性,采集所有患者空腹静脉血,采用酚-氯仿法从分离出的白细胞中提取脱氧核糖核酸(DNA),采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术分析HLA-B*1502的多态性,并比较其与芳香族致敏的发病风险及其治疗疗效的相关性。结果:通过对HLA-B*1502启动子区单核苷酸多态性的分析发现,经线性回归分析发现,携带CT/TT基因型较其他基因型的患者芳香族致敏发生率明显增高(P<0.05);通过对HLA-B*1502包含的1/1、1/2、1/3、1/4、2/2与4/4基因型的分析发现,以上6种基因型在试验组和对照组的分布差异无统计学意义(P>0.05),由主效应分析可知,变异型基因1/2与2/2可能降低芳香族致敏的发生率,但病例数较少,因此没有统计学意义(P>0.05);HLA-B*1502基因的多态性与芳香族致敏的临床治疗效果无明显相关性(P>0.05)。结论:携带CT/TT基因型的HLA-B*1502可能提高芳香族致敏的发病率,HLA-B*1502基因多态性与芳香族致敏的临床治疗效果不相关。Objective:The development of genetic medicine and preventive medicine has opened up a new field,aromatic sensitization in the body after the enzyme metabolism and protein polypeptide to form a semi-antigen complex,can induce later allergic reaction,this study analyzed the HLA-B*1502 gene polymorphism and the incidence of aromatic sensitization risk and therapeutic efficacy of the relationship.Methods:43 cases were selected as the test group,and 110 patients with the same health were selected as the control groups.The polymorphism of HLA-B*1502 gene was analyzed,the fasting venous blood was collected,and the DNA(DNA)was extracted from white cells separated by phenol-chloroform method.The polymorphism of HLA-B*1502 was analyzed by restrictive fragment length polymorphism polymerase chain reaction(PCR-RFLP),and its correlation with the risk of aromatic sensitization and its therapeutic efficacy was compared.Results:According to the analysis of single nucleotide polymorphism in HLA-B*1502 promoter region,it was found that the incidence of aromatic sensitization was significantly higher in patients with ct/tt genotype than in other genotypes(P<0.05);Through the HLA-B*1502 contained 1/1,1/2,1/3,1/4,2/2 and 4/4 genotype analysis showed that there was no statistically significant difference in the distribution of the above 6 genotypes in the test group and the control groups(P>0.05),and it was found from the main effect analysis that the variable-shaped gene 1/2 and 2/2 could decrease the incidence of aromatic sensitization,but the number of cases was less Therefore,there was no statistically significant(P>0.05),and there was no significant correlation between the polymorphism of HLA-B*1502 gene and the clinical efficacy of aromatic sensitization(P>0.05).Conclusion:The HLA-B*1502 with CT/TT genotype may improve the incidence of aromatic sensitization,and HLA-B*1502 gene polymorphism is not related to the clinical therapeutic effects of aromatic sensitization.

关 键 词：芳香族致敏 基因多态性 发病风险 预后效果 

分 类 号：R742.1[医药卫生—神经病学与精神病学]