RRM2对人胃癌细胞MKN-45增殖、侵袭及顺铂耐药作用的研究  

Study of RRM2 on Proliferation,Invasion and Cisplatin Resistance to MKN-45 Cells

作　　者：侯志斌 李菊英 苟笑丹[3] HOU Zhibin;LI Juying;GOU Xiaodan(Department of Pathology,Xining No.2 People′s Hospital,Xining 810003,China;Department of Pathology,Qinghai University,Xining 810016,China;Department of Pathology,Qinghai University Affiliated Hospital,Xining 810001,China)

机构地区：[1]西宁市第二人民医院病理科,西宁810003 [2]青海大学病理学教研室,西宁810016 [3]青海大学附属医院病理科,西宁810001

出　　处：《医学综述》2018年第13期2684-2688,共5页Medical Recapitulate

基　　金：西宁市卫生计生委指导性科技项目计划(2016009)

摘　　要：目的研究核糖核苷酸还原酶亚单位M2(RRM2)基因在人胃癌MKN-45细胞增殖、侵袭、耐药中的作用,并初步探讨其可能的机制。方法设计两组靶向RRM2基因的小干扰RNA,分别转染胃癌细胞MKN-45以抑制RRM2基因的表达,并同时设立阴性对照组(NC组)及未转染组。转染后琼脂糖电泳检测各组细胞RRM2基因表达,Western-blot检测各组细胞RRM2蛋白表达。CCK-8法对各组细胞增殖能力进行检测。不同浓度顺铂处理各组细胞进行耐药能力检测。采用Transwell小室对各组细胞侵袭能力进行检测。结果琼脂糖电泳及Western-blot检测显示转染RRM2-siRNA后,RRM2在基因水平及蛋白水平表达均明显下调。转染RRM2-siRNA的人胃癌MKN-45细胞的增殖能力、侵袭能力均显著低于未转染组及NC组(P<0.05),转染组顺铂IC50分别为0.081μg/m L及0.079μg/m L,明显低于未转染组(0.14μg/m L)及NC组(0.136μg/m L)(P<0.01)。结论 RRM2基因具有促进胃癌细胞增殖、侵袭的作用,并与顺铂耐药相关。Objective To explore the role of ribonucleotide reductase M2(RRM2)gene on proliferation,invasion and drug resistance in MKN-45 gastric cancer cells and primarily discuss the relative mechanisms.Methods Two RRM2-siRNA were used to transfect MKN-45 gastric cancer cells and negative control(NC)group and parent group were established as well.Agarose electrophoresis and Western-blot were used to detect expression of RRM2 at gene and protein level respectively.The change of proliferation was detected by CCK-8 method.Drug resistance was detected by cisplatin of different concentrations.Transwell chamber was used to detect the cell invasive ability.Results Expression of RRM2 at gene and protein level decreased obviously after transfection with RRM2-siRNA.The proliferation,invasion were significantly lower than the parent group and NC group(P<0.05).IC 50 inhibited of the transfected group was 0.081μg/mL and 0.079μg/mL,signficantly lower than the NC group(0.136μg/mL)and parent group(0.14μg/mL)(P<0.01).Conclusion RRM2 gene promotes proliferation,invasion of gastric cancer cells,and is associated with the resistance to cisplatin.

关 键 词：胃癌 核糖核苷酸还原酶亚单位M2 小干扰RNA 

分 类 号：R735.2[医药卫生—肿瘤]