贝伐单抗与胃癌细胞增殖、凋亡及HER-3蛋白表达的相关性研究  

Study on the Relationship between Bevacizumab with Gastric Cancer Cell Proliferation,Apoptosis and HER-3 Protein Expression

作　　者：陈睿 宁泽 陈柯宏 张成宇 杨永胜 付本伟 CHEN Rui;NING Ze;CHEN Kehong;ZHANG Chengyu;YANG Yongsheng;FU Benwei(Department of Oncology and Hematology,Hospital of Chongqing Red Cross Society/Jiangbei District People′s Hospital,Chongqing 400020,China;Comprehensive Experimental Research Center of Southwest Hospital Affiliated to Army Medical University,Chongqing 400038,China)

机构地区：[1]重庆市红十字会医院(江北区人民医院)肿瘤血液科,重庆400020 [2]陆军军医大学附属西南医院综合实验研究中心,重庆400038

出　　处：《医学综述》2018年第13期2693-2696,2700,共5页Medical Recapitulate

摘　　要：目的探讨贝伐单抗对体外培养SGC-7901人胃癌细胞株增殖、凋亡的影响及与人类表皮生长因子3(HER-3)蛋白表达的相关性。方法用不同浓度的贝伐单抗作用于SGC-7901人胃癌细胞,经24、48、72 h作用后,采用四甲基偶氮唑盐比色法分别检测胃癌细胞抑制率;应用流式细胞术分析贝伐单抗对SGC-7901人胃癌细胞细胞凋亡及周期分布的影响;应用免疫印迹法检测贝伐单抗处理后HER-3蛋白的表达水平。结果随着浓度的升高和作用时间的增加,贝伐珠单抗对SGC-7901细胞增殖的抑制率作用增强,除空白对照组外,其他三组的细胞抑制率在48 h和72 h明显大于24 h,各组间、时点间、组间和时点间交互作用比较差异均有统计学意义(P<0.01)。各组贝伐单抗处理后SGC-7901细胞停留在G1期、S期的细胞比例和凋亡率比较差异有统计学意义(P<0.01)。空白对照组、2、4、8 mg/L处理后HER-3的相对表达量分别为0.81±0.07、0.63±0.06、0.44±0.06、0.21±0.04,各组比较差异有统计学意义(P<0.001)。结论贝伐单抗可以抑制SGC-7901人胃癌细胞增殖、诱导其凋亡,HER-3蛋白表达水平与贝伐单抗浓度呈负相关。Objective To investigate the effect of bevacizumab on the proliferation and apoptosis of SGC-7901 human gastric cancer cells in vitro and the correlation with the expression of human epidermal growth factor receptor-3(HER-3)protein.Methods With the effect of different concentrations of bevacizumab in SGC-7901 human gastric cancer cells,by 24,48,72 h after,gastric cancer cell inhibition rate was detected with methyl thiazolyl tetrazolium method;analysis of bevac izumab effect on SGC-7901 human gastric cancer cell apoptosis and cell cycle distribution was done by flow cytometry;immune blotting was used to measure the protein expression level of HER-3.Results With the increase of the concentration and time,bevacizumab′s inhibitory effect on the proliferation of SGC-7901 cells was enhanced.Except for the blank control group,the cell inhibition at 48 h and 72 h of the other three groups was significantly greater than that at 24 h.There were statistically significant differences between groups,time points and time and group interaction(P<0.01).The cell ratio and apoptotic rate of SGC-7901 cells staying in G 1 and S phases after treatment with bevacizumab were statistically significant difference(P<0.01).The relative expression levels of HER-3 in the blank control group,and after 2,4,and 8 mg/L treatment were 0.81±0.07,0.63±0.06,0.44±0.06,and 0.21±0.04,respectively,and there was a significant difference between the groups(P<0.001).Conclusion Bevacizumab can inhibit the proliferation and induce apoptosis of SGC-7901 human gastric cancer cells,and the level of HER-3 protein expression is negatively correlated with the concentration of bevacizumab.

关 键 词：胃癌 贝伐单抗 表皮生长因子受体3 耐药 

分 类 号：R735.2[医药卫生—肿瘤]