2种降脂药物对高脂饮食金黄地鼠肝脏组织病理学影响比较研究  

Effects Comparison Between Simvastatin and Ezetimibe on Liver Histopathology in High-fat-fed Golden Hamsters Model

作　　者：曹晓东[1] 常福厚[1] CAO Xiao-dong;CHANG Fu-hou(Center for New Drugs Safety Evaluation and Research,Inner Mongolia Medical University,Inner Mongolia Engineering Research Center of New Drug Screening,Hohhot 010059,China)

机构地区：[1]内蒙古医科大学新药安全评价研究中心内蒙古自治区新药筛选工程研究中心,内蒙古呼和浩特010059

出　　处：《畜牧与饲料科学》2018年第7期23-26,共4页Animal Husbandry and Feed Science

摘　　要：旨在比较辛伐他汀与依折麦布对高脂饮食金黄地鼠降脂效果的差异。选取体况健康的雄性叙利亚金黄地鼠40只随机分为4组,分别为空白对照组、高脂模型组、辛伐他汀组和依折麦布组;空白对照组饲喂基础饲料,其他3组饲喂高脂饲料;采用灌胃方法分别给予高脂饲喂的金黄地鼠辛伐他汀与依折麦布,连续灌胃9周,空白对照组和高脂模型组同期灌胃蒸馏水;试验结束时,称量各组金黄地鼠体重,取肝脏样本进行称重,计算肝体比;制备肝脏组织学切片进行HE染色,利用显微镜观察病理变化。结果表明:与空白对照组相比,辛伐他汀组的肝脏重量极显著增加(P<0.01),依折麦布组的肝脏重量变化不显著(P>0.05);辛伐他汀组的肝体比极显著高于空白对照组(P<0.01),依折麦布组的肝体比显著低于空白对照组(P<0.05);辛伐他汀组肝脏颜色呈土黄色,表面有颗粒感,边缘锐利,眼观特征与高脂模型组相似;依折麦布组肝脏与空白对照组肝脏眼观特征一致,均呈暗褐色,质地柔软,边缘锐利;辛伐他汀组肝脏镜检观察可见肝周边缘部肝细胞质内出现大脂肪空泡,将细胞核挤向一边,可见空泡变性和炎性细胞浸润;依折麦布组肝脏组织学检查可见肝细胞均质红染,胞质丰富,未见大脂肪空泡、空泡变性和炎性细胞浸润,肝索排列整齐。综上提示,依折麦布对高脂饲喂的金黄地鼠肝脏的降脂效果优于辛伐他汀。The objective of this study was to characterize the difference in antihypercholesterolemic effect between simvastatin and ezetimibe in high-fat-fed golden hamsters model.A total of 40 healthy male golden hamster were randomly assigned to the following four groups:normal control group,hyperlipidemic model group,simvastatin group and ezetimibe group.The normal control group fed the normal diet and the other three groups fed a high-fat diet.The simvastatin group and ezetimibe group were intragastrically administrated 3 mg/(kg·BW)of simvastatin and 2.5 mg/(kg·BW)of ezetimibe for consecutive nine weeks,respectively,and the normal control group and hyperlipidemic model group were received distilled water contemporaneously.At the end of the experiment,the body weight of the golden hamsters was determined and their liver were sampled and weighted to calculate the ratio of liver weight to body weight;the histological sections of liver were prepared and were stained with HE method;the pathological changes of liver tissues were observed using light microscopy.The results showed that the simvastatin group had extremely significantly higher liver weight compared to normal control group(P<0.01),while the liver weight of ezetimibe group had no significant difference with normal control group(P>0.05);extremely significantly higher ratio of liver weight to body weight was observed in simvastatin group compared to normal control group(P<0.01),and the ratio of liver weight to body weight in ezetimibe group were found significantly reduced compared to normal control group(P<0.05);the simvastatin group had similar pathological changes of liver observed by naked eye to hyperlipidemic model group:the liver was earth yellow colored with granular feeling in surface and the edge was sharp;similar pathological changes of liver observed by naked eye were observed in ezetimibe group and normal control group:the liver was dark brown colored with soft texture and the edge was also sharp;under light microscopy,simvastatin group showed intracy

关 键 词：高脂饮食 肝脏 金黄地鼠 辛伐他汀 依折麦布 

分 类 号：S859.7[农业科学—临床兽医学] R96[农业科学—兽医学]