慢性乙型病毒性肝炎湿热类证潜在miRNA表达谱研究  

Study on expression profile of potential miRNA of chronic hepatitis B with dampness-heat syndrome

作　　者：苏悦[1] 罗雪梅 彭杨芷 党思捷[1] 刘西洋[1] 姜岑[1] SU Yue;LUO Xuemei;PENG Yangzhi;DANG Sijie;LIU Xiyang;JIANG Cen(Chengdu University of TCM,Chengdu,Sichuan 610075,China;T.C.M.Hospital of Ya′an,Ya′an,Sichuan 625000,China)

机构地区：[1]成都中医药大学,610075 [2]四川省雅安市中医院,625000

出　　处：《重庆医学》2018年第20期2663-2665,共3页Chongqing medicine

基　　金："十二五"传染病国家科技重大专项(2012ZX10005001);四川省中医药管理局项目(319787)

摘　　要：目的筛选慢性乙型病毒性肝炎(CHB)湿热类证潜在miRNA标志物。方法运用基因芯片技术,检测CHB脾胃湿热证组、肝胆湿热证组、脾虚湿热证组、隐证组及健康对照组的miRNA,分析获得湿热类证潜在miRNA标志物,并对其进行靶基因预测和功能分析。结果获得脾胃湿热证潜在miRNA表达谱9条、肝胆湿热证潜在miRNA表达谱14条、脾虚湿热证潜在miRNA表达谱17条;hsa-miR-1260a为脾胃湿热证、肝胆湿热证、脾虚湿热证共有的miRNA,且均表达上调,差异倍数分别是6.130 02、11.003 02、6.827 84。对其进行靶基因预测及功能富集分析,共获得3 302条靶基因,功能涉及肝素钠反应、Wnt信号通路等方面。结论CHB湿热类证潜在miRNA表达谱为hsa-miR-1260a。Objective To screen out the potential miRNA biomarker of chronic hepatitis B with dampness-heat syndrome patients.Methods Microarray technology was used to detect chronic hepatitis B miRNA in the spleen-stomach dampness-heat syndrome group,the liver and gallbladder dampness-heat syndrome group,the spleen deficiency and dampness-heat syndrome group,the hidden syndrome group and the control group,analyzed the potential miRNA markers of dampness-heat syndrome,and the target genes were predicted and the functions were analyzed.Results Nine potential mRNA biomakers were found in the spleen-stomach dampness-heat syndrome,14 potential mRNA biomakers were found in the liver and gallbladder dampness-heat syndrome,17 potential mRNA biomakers were found in the spleen deficiency and dampness-heat syndrome;hsa-miR-1260a was a mutual regulation miRNA among the three kinds of dampness-heat syndrome,and all expressions were up-regulated,the differences were 6.130 00,11.003 02,and 6.827 84 respectively.The target gene prediction and enrichment analysis showed that a total of 3 302 target genes were obtained,the functions involved heparin sodium response and Wnt signaling pathways.Conclusion The potential miRNA biomarker of chronic hepatitis B with dampness-heat syndrome is hsa-miR-1260a.

关 键 词：慢性乙型病毒性肝炎 湿热类证 MIRNA 生物学标志物 

分 类 号：R229[医药卫生—中医基础理论]