TBO-PDT对大鼠口腔混合菌生物膜抑制作用的实验研究  

The Experimental Study on the Inhibitory Effect of TBO-PDT on Biofilm of Oral Mixed Bacteria in Rats

作　　者：陶亚东 孙继红 柳雪[2] 王鹏[1] 霍峰[1] TAO Yadong(The Affiliated Hospital of Chengde Medical,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院,河北承德067000 [2]承德医学院,河北承德067000

出　　处：《河北医学》2018年第7期1187-1194,共8页Hebei Medicine

基　　金：2016年承德市科学技术研究与发展计划项目;(编号:201606A043)

摘　　要：目的:探讨不同光敏剂浓度和不同光照时间的TBO-PDT对大鼠口腔混合菌菌斑生物膜的抑制效果,确定最佳光敏剂参数和时间参数,并在此基础上探讨TBO-PDT在抑制混合菌菌斑生物膜的作用优势,为PDT应用于龋病预防提供理论与实验依据。方法:以Wistar大鼠构建混合菌致龋模型。将其随机分组:A组为生理盐水阴性处理组;B组为洗必泰阳性对照组;其余组别根据光敏剂浓度的不同,分为C1组60μg/m L、D1组80μg/m L、E1组100μg/m L、F1组120μg/m L、G1组140μg/m L,避光孵育5min后用波长为630nm,输出强度为105m W/cm2的二极管激光器照射9min;根据照射时间的不同,分为C2组3min、D2组6min、E2组9min、F2组12min、G2组15min,光敏剂浓度100mg/L避光孵育5min后用波长为630nm,输出强度为105m W/cm2的二极管激光器照射;实验处理后取样,通过平板菌落计数法观察各组对大鼠口腔混合菌生物膜的抑制作用,原子吸收分光光度计测定光动力疗法作用后大鼠口腔混合菌生物膜的钙离子溶出量,评价TBO-PDT对大鼠口腔混合菌菌斑生物膜的抑制作用。结果:不同光敏剂浓度下TBO-PDT对大鼠口腔混合菌菌斑生物膜抑菌效果的影响。平板菌落计数结果显示:不同光敏剂浓度的各组抑菌率随光敏剂浓度的增加而增高。与阴性对照组相比,C1组、D1组、E1组、F1组和G1组的抑菌率显著提高,E1组、F1组和G1抑菌率高于阳性对照组,而F1和G1两组间抑菌率没有明显差异。不同光照时间下TBO-PDT对大鼠口腔混合菌菌斑生物膜抑菌效果的影响。平板菌落计数结果显示:不同光照时间的各组抑菌率随时间的增加而增高。与阴性对照组相比,C2组、D2组、E2组、F2组和G2组的抑菌率显著提高,E2组、F2组和G2抑菌率高于阳性对照组,而F2和G2两组间抑菌率没有明显差异。原子吸收分光光度计测定钙离子溶出量结果显示,在48h内,各组钙离子溶出量(ug/mL)随时间的�Objective:To Study the inhibitory effect of TBO-PDT in different photosensitizer concentration and different lighting time on the cariogenic bacteria in dental plaque biofilms of rats,and determine the optimal photosensitizer parameters and time parameters.On the basis of this,to discuss the advantages of TBO-PDT in inhibiting the biofilm of mixed bacterial plaque,and provided theoretical and experimental evidence for the application of PDT in the prevention of dental caries and to provid theoretical and experimental basis for the application of PDT for dental caries prevention.Method:The caries model was constructed with Wistar rats.All the rats in accordance with the principle of randomization were divided into groups as follows:Group A:a saline-negative treatment group;Group B:a positive control group for chlorhexidine.The other groups were based on the concentration of the photosensitizer as group C1:60mg/L,group D1:80mg/L,group E1:100mg/L,group F1:120mg/L,group G1:140mg/L.Treated group C1-G1 with incubation for 5 minutes in dark place and exposed 9min by a semiconductor laser with a wavelength of 630nm.The output strength was 10 5 mW/cm 2.The rest groups were based on the exposure time:group C2:3min;group D2:6min;group E2:9min;group F2:12min;group G2:15min.Treated group C2-G2 with incubation for 5 minutes in dark place and exposed by a semiconductor laser with a wavelength of 532nm.The photosensitizer concentration was 100mg/L.The output strength was 10 5mW/cm 2.According to the different exposure time,it was divided into C2 group 3min,D2 group 6min,E2 group 9min,F2 group 12min,G2 group 15min,and the photosensitizer concentration was 100mg/L after incubating 5min,and the output intensity was 10 5 diode laser.The inhibitory effect of TBO-PDT on oral mixed bacteria biofilm of rat oral mixed bacteria was evaluated by atomic absorption spectrophotometer,and the inhibitory effect of TBO-PDT on oral mixed bacterial plaque biofilm was evaluated by atomic absorption spectrophotometer.Resultes:The bacteriostatic effe

关 键 词：光动力疗法 菌斑生物膜 抑菌率 

分 类 号：R780.2[医药卫生—口腔医学]