肝细胞癌患者血清和肝癌组织中T细胞免疫球蛋白粘蛋白分子-3表达及其意义  

Expression of T cell immunoglobulin domain and mucin domain protein-3 in serum and liver tissue of patients with hepatocellular carcinoma and its clinical significance

作　　者：李林株[1] 郭灵敏[1] 杜佩铭 康靖华[1] 吴海彬 Li Linzhu;Guo Lingmin;Du Peiming;Kang Jinhua;Wu Haibin(Department of Pathology,People's Hospital Gaoming District,Foshan 528500,China)

机构地区：[1]佛山市高明区人民医院病理科,528500

出　　处：《国际医药卫生导报》2018年第14期2110-2113,共4页International Medicine and Health Guidance News

摘　　要：目的 探讨Tim-3在肝细胞癌患者血清和肝癌组织中的表达及其临床意义。方法 应用酶联免疫吸附法(ELISA)和免疫组化方法检测肝细胞癌患者血清和肝癌组织中TIM-3的表达,分析TIM-3表达与患者临床病理特征的关系。结果 研究组入院时和术后1周血清TIM-3的表达分别为(47.36±8.14)pg/ml、(20.08±4.19)pg/ml,对照组血清TIM-3的表达为(11.27±4.16)pg/ml,研究组入院时血清TIM-3的表达浓度明显高于对照组(t=8.29,P<0.05),术后血清TIM-3的表达浓度显著降低,明显低于入院时的表达水平(t=6.94,P<0.05)。TIM-3蛋白在肝癌组织中的阳性表达率(88.23%)显著高于癌旁组织(24.71%),差异有统计学意义(χ2=69.78,P<0.05)。研究组入院时血清TIM-3表达浓度与AFP、TNM分期、门静脉癌栓以及淋巴结转移密切相关(P<0.05)。AFP>400μg/L、TNM分期为Ⅲ~Ⅳ、伴有淋巴结转移及门静脉癌栓的患者肝癌组织中TIM-3蛋白表达明显高于TNM分期为Ⅰ~Ⅱ、无淋巴结转移及门静脉癌栓的患者(P<0.05)。结论 TIM-3在HCC患者血清和肝癌组织中检测均异常表达,且其表达与患者的恶性进展存在密切相关性。Objective To study the expression of T cell immunoglobulin domain and mucin domain protein-3(TIM-3)in serum and liver tissue of patients with hepatocellular carcinoma(HCC)and its clinical significance.Methods The expression of TIM-3 in serum and liver tissue of patients with HCC were detect by enzyme-linked immunosorbent(ELISA)and immunohistochemical method;and the relationship between the expression of Tim-3 and the clinical pathological characteristics was analyzed.Results The serum expressions of TIM-3 in the study group were(47.36±8.14)pg/ml and(20.08±4.19)pg/ml on admission and after operation respectively,with a statistical difference(t=6.94,P<0.05),and(11.27±4.16)pg/ml in the control group;the serum expression of TIM-3 on admission of the study group was significantly higher than that of the control group(t=8.29,P<0.05).The positive expression rate of TIM-3 in hepatocellular carcinoma tissue was significantly higher than that in the tissue adjacent to carcinoma(88.23%vs.24.71%,χ2=69.78,P<0.05).The serum expression of TIM-3 significantly correlated with AFP,portal venous tumor emboli,TNM staging,and lymph node metastasis(P<0.05).AFP>400μg/L,the expression of TIM-3 protein in patients with TNM stageⅢ-Ⅳand associated with lymph node metastasis,and portal venous tumor emboli were significantly higher than those of patients with TNM stagingⅠ-Ⅱand without lymph node metastasis and portal venous tumor emboli(P<0.05).Conclusion The expression TIM-3 in the serum and liver tissue of patients with HCC is abnormal;and its expression closely correlates with malignant progression.

关 键 词：肝细胞癌 TIM-3 临床病理特征 

分 类 号：R735.7[医药卫生—肿瘤]