PCR-RFLP和PCR-SSCP在食源性感染致病菌检验中的应用  

Application of PCR-RFLP and PCR-SSCP in the inspection of foodborne pathogens

作　　者：谭炜炜[1] 董进浪[1] 吕建辉[1] Tan Weiwei;Dong Jinlang;Lyu Jianhui(Clinical Lab,Laiyang Central Hospital of Yantai City,Yantai 265200,China)

机构地区：[1]烟台市莱阳中心医院检验科,265200

出　　处：《国际医药卫生导报》2018年第13期1970-1973,共4页International Medicine and Health Guidance News

摘　　要：目的 探讨PCR-RFLP和PCR-SSCP在食源性感染致病菌检验中的应用。方法 将鉴别靶基因设定为食源性感染14种常见致病菌的23S rRNA基因,扩增时运用通用引物PCR(UP-PCR),并运用单链构象多态性分析(SSCP)和限制性酶切片段长度多态性分析(RFLP)分析PCR产物的酶切片段。结果 针对23S rRNA基因片段的Hpa II酶切产物的RFLP分析表明,不同种属的致病菌表现的RFLP图谱相似;SSCP分析显示,SSCP图谱具有较大的变异性,能够为细菌鉴定提供有效依据。结论 PCR-SSCP技术能够快速鉴定食源性感染致病菌,值得在临床推广应用。Objective To investigate the application of PCR-RFLP and PCR-SSCP in the inspection of foodborne pathogens.Methods 23S rRNA gene of 14 kinds of common foodborne pathogens were set as target genes,universal primers PCR(UP-PCR)was used in amplification,and restriction fragment length polymorphism(RFLP)analysis and single strand conformation polymorphism(SSCP)analysis were used to analyze PCR products digested fragments.Results RFLP for Hpa II digestion products 23S rRNA gene fragment analysis showed that RFLP pattern of different species of bacteria had similar performance;SSCP analysis showed that SSCP patterns had greater variability,so could provide an effective basis for the identification of bacteria.Conclusion PCR-SSCP technique can rapidly identify foodborne pathogens,so is worthy of promotion and application in the clinical.

关 键 词：PCR-RFLP PCR-SSCP 食源性感染致病菌 检验 应用 

分 类 号：R155.5[医药卫生—营养与食品卫生学]