和厚朴酚对组织因子途径抑制物-2诱导神经胶质瘤细胞凋亡的实验研究  

Experimental study of honokiol on apoptosis of glioma cells induced by tissue factor pathway inhibitor-2

作　　者：杜杰 杨勇波 郑刘芳 DU Jie;YANG Yongbo;ZHENG Liufang(Department of Neurology,the People’s Hospital of kaizhou District,CQ,Chongqing 405400,China)

机构地区：[1]重庆重庆市开州区人民医院神经内科,405400 [2]重庆市开州区人民医院肿瘤科,405400

出　　处：《临床肿瘤学杂志》2018年第7期582-586,共5页Chinese Clinical Oncology

摘　　要：目的探讨和厚朴酚对组织因子途径抑制物-2(TFPI-2)表达的影响,以及和厚朴酚和TFPI-2过表达对人胶质瘤U251细胞凋亡的影响。方法体外培养胶质瘤U87、U251、LN18、LN229、A172细胞系,荧光定量PCR(QPCR)检测不同细胞系中TFPI-2的表达。不同浓度(0、10、20、30、40和50μmol/L)和厚朴酚干预U251细胞,QPCR和Western blotting检测TFPI-2 mRNA和蛋白的表达。采用腺病毒载体p GSadeno-TFPI-2过表达U251细胞内TFPI-2的表达,流式细胞术及caspase-3试剂盒检测细胞凋亡率和caspase-3活性。结果在选取的5种不同胶质瘤细胞株中,U251和A172细胞内TFPI-2 mRNA明显下降(P<0.05)。和厚朴酚呈浓度依赖性增加U251细胞内TFPI-2 mRNA水平;其中50μmol/L和厚朴酚干预24 h后,TFPI-2 mRNA表达水平升高最明显(P<0.05)。腺病毒感染U251细胞后TFPI-2表达水平明显增加(P<0.05)。过表达TFPI-2和50μmol/L和厚朴酚干预均可增加U251细胞凋亡水平和caspase-3的活性(P<0.05)。结论和厚朴酚处理可增加U251细胞内TFPI-2的表达;和厚朴酚联合TFPI-2过表达显著诱导胶质瘤细胞凋亡。Objective To investigate the effect of honokiol on the expression of tissue factor pathway inhibitor-2(TFPI-2)and the effect of overexpression of honokiol and TFPI-2 on the apoptosis of human glioma U251 cells.Methods The U87,U251,LN18,LN229 and A172 cell lines of glioma were cultured in vitro,and the expression of TFPI-2 in different cell lines was detected by fluorescence quantitative PCR(QPCR).Honokiol treated U251 cells with different concentrations(0,10,20,30,40 and 50μmol/L).QPCR and Western blotting were used to detect the expression changes of TFPI-2 mRNA and protein.The expression of TFPI-2 in U251 cells was overexpressed by adenovirus vector pGSadeno-TFPI-2,and apoptosis rate and caspase-3 activity were detected by flow cytometry and caspase-3 kit.Results The TFPI-2 mRNA in U251 and A172 cells decreased significantly in U87,U251,LN18,LN229 and A172 cell lines(P<0.05).Honokiol increased TFPI-2 mRNA level in U251 cells in a concentration dependent manner(P<0.05).After 50μmol/L honokiol intervened for 24 h,the expression level of TFPI-2 mRNA increased most significantly(P<0.05).The expression level of TFPI-2 in U251 cells increased significantly after transfected with adenovirus vector pGSadeno-TFPI-2(P<0.05).Overexpression of TFPI-2 and 50μmol/L honokiol could increase U251 cell apoptosis and caspase-3 activity(P<0.05).Conclusion Honokiol can increase the expression of TFPI-2 in U251 cells,and overexpressed TFPI-2 combined with honokiol significantly induced apoptosis in glioma cells.

关 键 词：胶质瘤 组织因子途径抑制物-2 和厚朴酚 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]