脑胶质瘤组织中微小RNA-21水平及其与EGFR和VEGF表达的关系  

Analysis of microRNA-21 level in glioma tissues and its relationship with expressions of EGFR and VEGF

作　　者：张照楠 宿伟鹏[1] 张洋[1] 张宋安[1] 赵化荣[1] ZHANG Zhaonan;SU Weipeng;ZHANG Yang;ZHANG Songan;ZHAO Huarong(Cancer Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,乌鲁木齐830054

出　　处：《临床肿瘤学杂志》2018年第6期514-518,共5页Chinese Clinical Oncology

基　　金：乌鲁木齐市科学技术项目(Y141310033)

摘　　要：目的探讨脑胶质瘤组织中微小RNA-21(miR-21)水平及其与表皮生长因子受体(EGFR)和血管内皮生长因子(VEGF)表达的关系。方法选取本院2005年1月至2015年6月手术切除并经病理证实的WHOⅢ、Ⅳ级脑胶质瘤组织92例,另取50例癌旁组织作对照。采用实时荧光定量PCR(QPCR)检测以上组织中的miR-21水平,免疫组化检测EGFR和VEGF表达,分析脑胶质瘤组织中miR-21水平与临床病理参数(性别、年龄、民族和病理分级)及EGFR和VEGF表达的关系,Spearman等级相关分析miR-21水平与EGFR和VEGF表达的相关性。结果 QPCR检测结果显示脑胶质瘤组织中miR-21水平为130.7±76.9,高于癌旁组织,差异有统计学意义(P<0.05);miR-21水平与性别、年龄及民族无关(P>0.05),仅与病理分级有关(P<0.05),其中Ⅳ级的miR-21水平为164.83±49.80,高于Ⅲ级的32.29±31.65,差异有统计学意义(P<0.05)。EGFR和VEGF阳性表达率均为87.0%(80/92),其中EGFR阳性表达者的miR-21水平为139.52±80.62,高于阴性表达者的34.87±33.09(P<0.05),而VEGF阳性表达者的miR-21水平为138.74±83.40,高于阴性表达者的20.90±14.58(P<0.05);Spearman等级相关分析发现脑胶质瘤组织中miR-21水平与EGFR、VEGF表达均呈正相关(r=0.251、0.311,P<0.05)。结论脑胶质瘤组织中miR-21水平升高,且与病理分级和EGFR、VEGF表达有关,可能参与了该恶性肿瘤的发生发展,对脑胶质瘤的诊治有一定临床意义。Objective To investigate the level of microRNA-21(miR-21)in glioma tissues and its relationship with the expressions of epidermal growth factor receptor(EGFR)and vascular endothelial growth factor(VEGF).Methods Ninety-two cases of glioma tissues(WHOⅢandⅣgrade)surgically resected and confirmed by pathology in our hospital from January 2005 to June 2015 were collected.Another 50 cases of paracancerous tissues were taken as control.Real-time quantitative PCR(QPCR)was used to detect the level of miR-21 in the above tissues.Immunohistochemistry was used to detect the expressions of EGFR and VEGF.The relationships between miR-21 level and clinicopathological parameters(gender,age,nationality and pathological grade)as well as the expressions of EGFR and VEGF were analyzed in glioma tissues.Correlation between miR-21 level and expressions of EGFR and VEGF was analyzed by Spearman rank correlation analysis.Results QPCR detection showed that the miR-21 level in glioma tissues was 130.7±76.9,higher than that in adjacent tissues,and the difference was statistically significant(P<0.05).The level of miR-21 was not related to sex,age and nationality(P>0.05),only related to the pathological grade(P<0.05).The level of miR-21 of gradeⅣwas 164.83±49.80,higher than 32.29±31.65 of gradeⅢ,and the difference was statistically significant(P<0.05).The positive expression rates of EGFR and VEGF were both 87%(80/92).The miR-21 level in EGFR positive ones was 139.52±80.62,higher than 34.87±33.09 of the negative ones(P<0.05);the miR-21 level of the VEGF positive ones was 138.74±83.40,higher than 20.90±14.58 of the negative ones(P<0.05).Spearman rank correlation analysis showed that the miR-21 level in glioma tissues was positively correlated with the expressions of EGFR and VEGF(r=0.251,0.311,P<0.05).Conclusion The level of miR-21 in glioma tissues was elevated,which was related to the pathological classification and the expression of EGFR and VEGF.It may be involved in the development of the malignant tumor,and it has certain

关 键 词：脑胶质瘤 微小RNA-21 表皮生长因子受体 血管内皮生长因子 

分 类 号：R739.41[医药卫生—肿瘤]