厚朴酚抑制变形链球菌及釉质碳酸脱矿的研究  

Inhibition Effects of Magnolol on Streptococcus Mutans and Demineralized Enamel caused by Carbonated Beverages

作　　者：孔晶晶[1] 梁向阳[1] 李春年[1] 代鑫鹏 马向涛 KONG Jing-jing;LIANG Xiang-yang;LI Chun-nian;DAI Xin-peng;MA Xiang-tao(Hospital of Stomatology,Hebei Medical University,The Key Laboratory of Stomatology,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学口腔医院/河北省口腔医学重点实验室,河北石家庄050017

出　　处：《口腔医学研究》2018年第7期788-792,共5页Journal of Oral Science Research

基　　金：河北省中医药类科研计划课题(编号:2017177)

摘　　要：目的:观察变形链球菌对碳酸饮料导致的脱矿牙釉质的脱矿加速情况,通过加入厚朴酚液,抑制变形链球菌进而减慢釉质块的脱矿情况,为临床中碳酸饮料所致牙釉质脱矿的患者,减缓牙釉质继续脱矿致龋选择疗效较好的药物治疗提供实验依据和参考。方法:将因正畸拔除的前磨牙截根,将牙冠制备成5mm×5mm×2mm的近远中釉质块,每日置于碳酸饮料雪碧中30min×4次,每次间隔2h,连续3周,制备人工脱矿模型。将脱矿后的釉质块置于人工唾液中37℃恒温水浴,使之形成获得性膜。所有釉质块随机分为生理盐水组(A组),变形链球菌混悬液组(B组),菌液A值=1,变形链球菌混悬液+厚朴酚组(C组),厚朴酚浓度为6.3mg/L,每组20个,于37℃厌氧箱培养3周后,显微硬度仪检测牙釉质表面硬度,扫描电镜观察牙釉质表面,激光共聚焦显微镜观察牙釉质的荧光图像。结果:A、B、C三组釉质块脱矿后较脱矿前显微硬度值明显降低,差异均具有统计学意义(P<0.05);药物处理后,A组和C组釉质块显微硬度值无显著差异,且两组数值明显高于B组,差异均具有统计学意义(P<0.05)。扫描电镜观察B组釉质表面粗糙,大量釉柱断裂形成大小不等的孔隙,大量细菌附着,A组釉质表面部分孔隙变浅甚至消失,C组釉质表面孔隙明显减少,少量细菌附着。激光共聚焦显微镜观察B组荧光染料沉积多,荧光面积和荧光强度大,A组和C组荧光染料沉积较B组少,荧光面积和荧光强度小。结论:变形链球菌能够加速碳酸性脱矿牙釉质的脱矿速度;厚朴酚能够通过抑制变形链球菌,减慢碳酸性脱矿牙釉质的脱矿速度,进而减缓龋病的进展。Objective:To observe the promoting effect of streptococcus mutans on demineralized enamel caused by carbonated beverages and the inhibition effect of magnolol on streptococcus mutans to reduce the demineralization of enamel blocks.Methods:Extracted premolars without roots for orthodontic purpose were selected.All the crowns were divided into mesial and distal enamel blocks that were prepared into 5 mm×5 mm×2 mm.These enamel blocks were soaked in sprite for 30 minutes×4 times,2 hours at a time for 3 weeks to establish the demineralization model.The demineralized enamel blocks were placed in artificial saliva at 37℃room temperature water bath to form the acquired pellicle.Then they were randomly divided into normal saline group(group A),streptococcus mutans suspension group(group B,OD of the bacterial suspension was 1),streptococcus mutans suspension+magnolol group(group C,the concentration of magnolol was 6.3mg/L).All enamel blocks were placed in anerobic box at 37℃for 3 weeks.The microhardness value was tested by microhardness.The enamel surfaces were observed under scanning electron microscope.The fluorescence images were taken under the laser scanning confocal microscope.Results:After demineralization,the microhardness value of the three group were lower than that of before demineralization(P<0.05).The microhardness value of group A and group C was not different from that of before medication,however,higher than that of group B(P<0.05).Under scanning electron microscope,the enamel surfaces of group B were rough and the unequal size pore that formed by a great deal of fractured enamel rod,and a large number of bacteria attached.Enamel surfaces of group A showed some pores became shallow or disappeared.Enamel surfaces of group C pores significantly reduced,and a few bacteria attached.Under the laser scanning confocal microscope,the fluorescence area and intensity of group B were strong,the fluorescence area and intensity of group A and group C were lower than that of group B.Conclusion:Streptococcus mutans

关 键 词：厚朴酚 变形链球菌 牙釉质碳酸脱矿 抑制作用 

分 类 号：R780.2[医药卫生—口腔医学]