Hcy代谢相关基因甲基化与脑梗死的相关关系  

The relationship between Hcy metabolism related genes methylation and cerebral infarction

作　　者：李晓东 段世伟[2] 曲坤 白宏英[1] 肖健豪 袁倩 王运良[3] LI Xiaodong;DUAN Shiwei;QU Kun;BAI Hongying;XIAO Jianhao;YUAN Qian;WANG Yunliang(Department of Neurology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014,China;School of Medicine,Ningbo University,Ningbo 315211,China;Department of Neurology,the 148th of Hospital of PLA,Zibo 255330,China)

机构地区：[1]郑州大学第二附属医院神经内科,河南郑州450014 [2]宁波大学医学院,浙江宁波315211 [3]中国人民解放军第148医院神经内科,山东淄博255300

出　　处：《中国实用神经疾病杂志》2018年第13期1424-1430,共7页Chinese Journal of Practical Nervous Diseases

摘　　要：目的评价Hcy代谢相关基因S-腺苷高半胱氨酸水解酶基因(AHCY)、胱硫醚β合成酶基因(CBS)甲基化与脑梗死之间有无相关关系。方法应用荧光定量甲基化特异性PCR(qMSP)检测研究对象外周血中AHCY、CBS基因甲基化水平,并分析其与脑梗死的相关关系。结果 (1)病例组AHCY甲基化水平为0.16%(0.07%,0.32%),对照组为0.25%(0.02%,0.67%),差异无统计学意义(P=0.115,Z=-1.577)。(2)病例组CBS甲基化水平70.20%(49.19%,94.87%),对照组为104.10%(74.65%,132.30%),差异有统计学意义(P=3.71E-10,Z=-6.266)。按性别、年龄进行亚组分析,CBS基因甲基化水平均低于对照组,且差异具有统计学意义(P<0.05)。结论 AHCY基因甲基化与脑梗死发病不相关,而CBS基因低甲基化与脑梗死的发病相关。Objective In order to evaluate relationship of Hcy metabolism related genes the S adenosylhomocysteine hydrolase gene(AHCY)and cystathionineβsynthase gene(CBS)methylation levels with cerebral infarction.Methods AHCY,CBS methylation levels were measured in peripheral blood of subjects using quantitative methylation specific polymerase chain reaction(qMSP)method.We analyzed the correlation of AHCY,CBS methylation levels with cerebral infarction.Results (1)There was no significant difference of AHCY methylation in CI and paired normal controls(0.16%(0.07%,0.32%)versus 0.25%(0.02%,0.67%),P=0.115,Z=-1.577).(2)There was a significant difference of CBS promoter methylation levels in CI and paired normal controls(70.20%(49.19%,94.87%)versus 104.10%(74.65%,132.30%),P=3.71E 10,Z=-6.266).Subgroup analysis by gender and age showed that the methylation level of CBS was statistically lower in the case than that in control group(P<0.05).Conclusion Our study indicated that the methylation of the AHCY may be not related to cerebral infarction,and the hypomethylation of CBS promoter was associated with the pathogenesis of cerebral infarction.

关 键 词：脑梗死 DNA甲基化 同型半胱氨酸 S-腺苷高半胱氨酸水解酶 胱硫醚Β合成酶 

分 类 号：R743.33[医药卫生—神经病学与精神病学]