LL-37对大鼠移植供肝缺血/再灌注损伤保护作用的研究  

Protective effects of LL-37 on ischemia reperfusion injury in rat liver transplantation

作　　者：张德林[1] 赵鹏伟[1] 刘江伟[1] 冯新富[1] ZHANG De-lin;Zhao Peng-wei;LIU Jiang-wei;FENG Xin-fu(Department of hepatobiliary surgery,Guizhou Provincial People’s Hospital,Guiyang Guizhou 550001,China)

机构地区：[1]贵州省人民医院肝胆外科,贵州贵阳550001

出　　处：《临床医药文献电子杂志》2018年第51期12-14,共3页Electronic Journal of Clinical Medical Literature

基　　金：贵州省卫生厅基金资助项目(项目编号:黔卫发[2005]138号文);贵州省科技创新人才团队基金(项目编号:黔科合人才团队[2014]4008号)

摘　　要：目的探讨抗菌肽LL-37对大鼠移植肝缺血再灌注损伤模型的预处理效果。方法采用Kamada-Calne双袖套法建立大鼠缺血再灌注原位肝移植模型,将SD大鼠随机分成生理盐水组、UW液处理组和UW液+LL-37处理组,各组分别检测移植肝再灌注后2 h、6 h和24 h血清AST和ALT,检测移植肝再灌注后24 h肝组织中炎症介质TNF-α和IL-10 m RNA及蛋白的表达。结果 UW液+LL-37处理组血清ALT和AST在各时间点均比UW液组及生理盐水组明显减低,再灌注24 h后肝组织TNF-αm RNA的表达比UW液组减低,TNF-α蛋白的表达比UW液组及生理盐水组均减低(P<0.05),而IL-10 m RNA及蛋白的表达比UW液组及生理盐水组均升高(P<0.05)。结论抗菌肽LL-37可能通过上调抗炎因子IL-10,下调促炎因子TNF-α减轻大鼠移植肝缺血再灌注损伤,达到保护供肝的作用。Objective To study the preconditioning effect of LL-37 on ischemia-reperfusion injury model of rat liver graft.Methods To establish ischemia reperfusion rat model of orthotopic liver trans-plantation using Kamada-Calne double cuff method,SD rats were randomly divided into saline group,UW treatment group and UW+LL-37 treatment group,the serum contentrations of AST and ALT were determined at 2h,6 h and 24 h after the schemia-reperfusion,and the liver tissue were sampled to observe the mRNA and protein expression of TNF-αand IL-10 at 24h after the schemia-reperfusion.Results The levels of serum ALT,AST and the protein expression of TNF-αin the UW+LL-37 treatment group were significantly lower than those in the UW group and saline group,the mRNA expression of TNF-αin the UW+LL-37 treatment group were significantly lower than the UW group,and the content of IL-10 in liver tissue was higher than that in the UW group and saline group(P<0.05).Conclusion LL-37 may reduce the ischemia-reperfusion injury of rat liver by raise the anti-inflammatory factor IL-10 and reduce the proinflammatory factor TNF-α,so as to protect the donor liver.

关 键 词：肝移植 缺血再灌注损伤 抗菌肽 

分 类 号：R735.7[医药卫生—肿瘤]