载地塞米松中空碳羟基磷灰石微球的合成与体外释放研究  

Synthesis and in vitro release of dexamethasone from hollow carbonated hydroxyapatite microspheres

作　　者：张雪[1] 赵旭[2] 李毅[1] 王哲[1] 张梦霖 卢金金 Zhang Xue;Zhao Xu;Li Yi;Wang Zhe;Zhang Menglin;Lu Jinjin(Department of Pedodontics,School and Hospitatal of Stomatology,Jilin University;School of Chemistry,Jilin University,Changchun 130021)

机构地区：[1]吉林大学口腔医学院儿童口腔科 [2]吉林大学化学学院,长春130021

出　　处：《口腔材料器械杂志》2018年第3期128-133,149,共7页Chinese Journal of Dental Materials and Devices

基　　金：吉林省自然科学基金(编号:20150101204JC);吉林省卫生计生委基金(编号:2014Z064)

摘　　要：目的制备碳羟基磷灰石中空微球,构建地塞米松药物缓释系统,为其作为活髓保存剂应用于临床提供实验数据。方法以甘氨酸(Gly)和十二烷基磺酸钠(SDS)组成的"核-壳"式复合物为模板,合成碳羟基磷灰石中空微球。利用场发射扫描电镜(FESEM)、高分辨透射电子显微镜(HRTEM)、X射线衍射仪(XRD)、氮气吸附脱附分析(BET)分别对其形貌、物相、比表面积及孔径进行表征分析。通过与无Gly组对比,探讨中空微球的形成机理。将地塞米松载入碳羟基磷灰石中空微球构成体外缓释系统,计算载药率、包封率和药物体外缓释时间。结果成功制备直径为2~4μm针状中空碳羟基磷灰石微球,该微球具有良好的载药性(载药率12.1%~34.8%)和体外药物缓释性能,缓慢释放达30天,几乎无突释现象。结论合成的碳羟基磷灰石中空微球类似骨和牙齿的无机物主要组成成分,载药率和包封率均较高,微球中药物缓慢释放接近线性。Objective To prepare hollow carbonated hydroxyapatite microspheres loaded with dexamethasone to develop a drug delivery system,thereby laying the scientific foundation for the clinical application of preservative for live pulp.Methods Hollow microspheres of carbon-hydroxyapatite were synthesized by using the“core-shell”complex of glycine(Gly)and sodium dodecyl sulfate(SDS).The morphology,phase,specific surface area and pore size were characterized by FESEM,HRTEM,XRD and BET,respectively.By comparing to the Gly-free group,the formation mechanism of hollow microspheres was explored.Dexamethasone was loaded into hollow carbonated hydroxyapatite microspheres to form an in vitro sustained-release system.The drug-loading rate,encapsulationt efficiency and drug sustained-release time in vitro were calculated.Results The prepared hydroxyapatite was a hollow microsphere composed of acicular nanoparticles with a diameter of 2-4μm.The hollow microspheres showed a good drug loading(drug loading rate of 12.1%~34.8%)effect and the release of dexamethasone initially increased linearly with time,with sustained release having been achievethe main inorganic components of bone and tooth,can be synthesized by using the organic matter(Gly)and the surfactant(SDS)as template.Loading rate and encapsulation efficiency of the drug are high,and in vitro release of the drug is nearly linear.d for almost 30 days with minimal burst phenomenon.Conclusion Hollow carbonated hydroxyapatite microspheres,similar to

关 键 词：碳羟基磷灰石 微球 地塞米松 体外缓释 

分 类 号：R783[医药卫生—口腔医学]