白细胞介素1β与机械牵拉共同作用角膜成纤维细胞CollagenⅠ及Lumican的表达  

Combined effects of interleukin 1 beta and mechanical stretching on Collagen Ⅰ synthesis and Lumican expression in corneal fibroblasts

作　　者：冯鹏飞[1] 李晓娜[1] 容烁[1] 陈维毅[1] 王晓君[1] Feng Peng-fei;Li Xiao-na;Rong Shuo;Chen Wei-yi;Wang Xiao-jun(Key Laboratory of Material Intensity and Structural Impact in Shanxi Province,Institute of Applied Mechanics and Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,Shanxi Province,China)

机构地区：[1]太原理工大学应用力学与生物医学工程研究所,山西省材料强度与结构冲击重点实验室,山西省太原市030024

出　　处：《中国组织工程研究》2018年第25期4077-4082,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(11572213,31271005,11402161,11402162);山西省研究生教育创新项目(2015BY28)。

摘　　要：背景:继发性圆锥角膜是角膜屈光手术后的并发症之一,但其发生机制尚不清楚。目的:探讨白细胞介素1β与机械牵拉对角膜成纤维细胞CollagenⅠ和Lumican表达的影响。方法:体外培养角膜成纤维细胞,对其施加不同幅度(5%,10%,15%)的机械牵拉和不同质量浓度的白细胞介素1β,共同作用12,24,36 h,通过实时荧光定量PCR检测CollagenⅠ和Lumican m RNA的表达变化。结果与结论:(1)白细胞介素1β单独作用12 h使CollagenⅠα1的表达降低(P<0.05),作用24 h使Lumican的表达升高(P<0.05);(2)5%牵拉单独作用24,36 h使CollagenⅠα1表达升高(P<0.05);10%和15%牵拉单独作用12,24,36 h使CollagenⅠα1和CollagenⅠα2的表达降低(P<0.05);(3)5%牵拉单独作用12 h使Lumican表达升高(P<0.05),而10%和15%牵拉单独作用12 h使Lumican表达降低(P<0.05);5%,10%,15%牵拉24 h和36 h时使Lumican表达升高(P<0.05);(4)白细胞介素1β和机械牵拉共同作用24,36 h使CollagenⅠα1和CollagenⅠα2表达降低,Lumican表达升高;(5)结果提示,白细胞介素1β能抑制CollagenⅠ的合成,而促进Lumican的表达。低幅度机械牵拉能促进角膜成纤维细胞胶原的合成,中高幅度机械牵拉抑制其胶原的合成。两者共同作用能抑制角膜成纤维细胞胶原合成且促进Lumican的表达。BACKGROUND:Secondary keratoconus is one of the complications after corneal refractive surgery,but the mechanism remains unclear.OBJECTIVE:To study the effects of interleukin-1βand mechanical stretching on the synthesis of the Collagen I and Lumican in corneal fibroblasts.METHODS:Rabbit corneal fibroblasts were cultured in vitro and were treated by different concentrations of interleukin-1βand different amplitudes of mechanical stretching(5%,10%,15%)for 12,24 or 36 hours.The expression of Collagen I and Lumican was detected by real-time fluorescent quantitative PCR.RESULTS AND CONCLUSION:Interleukin-1βdecreased the expression of Collagen Iα1 at 12 hours and increased the expression of Lumican at 24 hours(P<0.05).5%stretching alone increased the expression of Collagen Iα1 at 24 and 36 hours(P<0.05),but 10%and 15%stretching alone decreased the expression of Collagen Iα1 and Collagen Iα2 at 12,24 and 36 hours(P<0.05).5%stretching alone increased the expression of Lumican at 12 hours(P<0.05),while 10%and 15%stretching alone decreased the expression of Lumican at 12 hours(P<0.05).Moreover,5%,10%and 15%stretching alone increased the expression of Lumican at 24 and 36 hours(P<0.05).Reduced expression of Collagen Iα1 and Collagen Iα2 and increased expression of Lumican in corneal fibroblasts were observed at 24 and 36 hours after combined treatment with interleukin-1βand mechanical stretching.These results reveal that interleukin-1βcan inhibit the synthesis of Collagen I and promote the expression of Lumican.Low-amplitude stretching can increase collagen synthesis;however,middle-to-largeamplitude stretching can inhibit its expression in corneal fibroblasts.The synthesis of collagen can be suppressed and the expression of Lumican can be increased under the combined use of interleukin-1βand stretching.

关 键 词：角膜 成纤维细胞 白细胞介素1Β 机械牵拉 CollagenⅠ LUMICAN 国家自然科学基金 角膜 成纤维细胞 白细胞介素1Β 胶原Ⅰ型 组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]