三叶青原花青素结构分析  

Structural Analysis of Proanthocyanidins from Tetrastigma hemsleyanum Diels et Gilg

作　　者：李鑫[1] 刘景玲[1] 李彦[1] 郭万里[2] 王大巾[2] 梁宗锁[1,2] LI Xin;LIU Jingling;LI Yan;GUO Wanli;WANG Dajin;LIANG Zongsuo(College of Life Sciences,Northwest A&F University,Yangling 712100,China;College of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]浙江理工大学生命科学学院,浙江杭州310018

出　　处：《食品科学》2018年第16期247-255,共9页Food Science

基　　金：陕西省重大产业集群项目(2012KTCL02-07);"十二五"国家科技支撑计划项目(2015BAC01B03)

摘　　要：利用核磁共振碳谱、反相高效液相色谱-电喷雾质谱联用技术联合基质辅助激光解析离子化飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF MS)技术对三叶青根、茎及叶原花青素进行结构分析。结果表明,三叶青原花青素主要由原花青定和原翠雀定构成,末端单元以儿茶素为主,延伸单元以表儿茶素和表没食子儿茶素为主。三叶青根、茎及叶原花青素的平均聚合度分别为4.89±0.33、3.77±0.19及4.45±0.48。利用MALDI-TOF MS检测出三叶青根、茎原花青素的三聚体到十聚体,叶原花青素的三聚体到十一聚体。每个聚体内部普遍存在(表)儿茶素和(表)没食子儿茶素结构单元,没食子酰基化以及A型连接。三叶青根、茎及叶原花青素在结构上具有一定的相似性。本研究结果可为三叶青原花青素的基础研究和开发利用提供一定参考。The structures of proanthocyanidins from the root,stem and leaves of Tetrastigma hemsleyanum Diels et Gilg were analyzed with 13C nuclear magnetic resonance(13C NMR),reversed-phase high performance liquid chromatography electrospray ionization mass spectrometry(RP-HPLC-ESI-MS)and matrix-assisted laser desorption/ionization time-offlight mass spectrometry(MALDI-TOF MS).The results showed that the proanthocyanidins from T.hemsleyanum were mainly composed of procyanidin(PC)and prodelphinidin(PD).The terminal units were mainly composed of catechin while epicatechin and epigallocatechin were the dominant extension units.The mean degree of polymerization(mDP)of proanthocyanidins from the root,stem and leaves of T.hemsleyanum were found to be 4.89±0.33,3.77±0.19 and 4.45±0.48,respectively.Trimers to decamers of proanthocyanidins were detected from the root and stem,while trimers to undecamers of proanthocyanidins from the leaves of T.hemsleyanum by MALDI-TOF MS.(epi)Catechin and(epi)gallocatechin monomer units individually and together with galloyl substituents and A-type linkages prevalently existed in each polymeric cluster.The proanthocyanidins from the root,stem and leaves of T.hemsleyanum were structurally similar.The results of this study may provide useful data for the fundamental study and further development of T.hemsleyanum.

关 键 词：三叶青 原花青素 核磁共振碳谱 反相高效液相色谱-电喷雾质谱联用技术 基质辅助激光解析离子化飞行时间质谱 

分 类 号：O629.9[理学—有机化学]