木质素降解放线菌的鉴定及产酶特性研究  

Indentification and enzyme-production characteristics of lignin-degrading actinomyces

作　　者：马英辉[1,2] 李利军 卢美欢[1] 丁慧[3] 贺建超 MA Yinghui;LI Lijun;LU Meihuan;DING Hui;HE Jianchao(Shaanxi Province Institute of Microbiology,Xi’an Shaanxi 710043;College of Natural Resources and Environment,North West A&F University,Yangling Shaanxi 712100;Xi’an Supervision&Inspection Institute of Product Quality,Xi’an Shaanxi 710098)

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]西北农林科技大学资源环境学院,陕西杨凌712100 [3]西安市产品质量监督检验院,陕西西安710098

出　　处：《环境污染与防治》2018年第8期902-906,912,共6页Environmental Pollution & Control

基　　金：陕西省科学院应用基础研究基金资助项目(No.2015K-14);陕西省科技厅农业创新与公关项目(No.2016NY-197)

摘　　要：为获得具有木质素降解功能的放线菌菌株并研究其产酶特性,通过苯胺蓝平板和木质素平板从造纸厂污泥中分离得到一株木质素降解放线菌AG-11,其对木质素的降解率可达60%以上,经过16SrDNA测序鉴定为灰略红链霉菌(Streptomyces griseorubens),Genbank登陆号为KX774674。研究了AG-11在液体条件下的产酶特性,发现AG-11对碱性木质素的降解主要依靠胞外漆酶(Lac)和胞外木质素过氧化物酶(LiP),且产酶活性受到木质素降解中间产物的诱导,同时对金属离子表现出了较强的敏感性,氧化胁迫也能显著提高AG-11的产酶活力,在20mmol/L H_2O_2胁迫下,其胞外Lac及胞外LiP酶活可分别达25.42、26.81U/mL。In order to research the enzyme-production characteristics of lignin-degrading antinomyces,a lignin-degradation actinomycetes named as AG-11 was isolated from active-sludge by aniline blue plate and lignin plate method,its lignin degradation rate could reach to over 60%.The strain AG-11 was identified as Streptomyces griseorubens by 16S rDNA,and the Genbank accession number was KX774674.The enzyme-production characteristics showed that degradation of lignin mainly depended on extracellular laccase and lignin peroxidase from AG-11.Then enzyme-production ability was regulated by molecules inducer and was sensitive to metal ions.Strain AG-11 could significantly improve enzyme-producing ability under the oxidative stress,under 20 mmol/L of hydrogen peroxide,the extracellular laccase activity and lignin peroxidase activity were 25.42,26.81 U/mL respectively.

关 键 词：木质素 放线菌 漆酶 木质素过氧化物酶 降解 

分 类 号：X172[环境科学与工程—环境科学] X703