青钱柳和桑叶配伍组方的降血糖作用  

Hypoglycemic effect of medicinal formula composed of Cyclocarya paliurus and Mulberry leaves

作　　者：盛雪萍 赵梦鸽 蒋翠花[2] 张健[2] 殷志琦[1] SHENG Xueping;ZHAO Mengge;JIANG Cuihua;ZHANG Jian;YIN Zhiqi(Department of TCMs Pharmaceuticals&State Key Laboratory of Natural Medicines,School of Traditional Chinese Medicine,China Pharmaceutical University,Nanjing 210009;Laboratory of Translational Medicine,Jiangsu Provincial Academy of Traditional Chinese Medicine,Nanjing 210028,China)

机构地区：[1]中国药科大学中药学院中药制剂系&天然药物活性组分与药效国家重点实验室,南京210009 [2]江苏省中医药研究院转化医学实验室,南京210028

出　　处：《中国药科大学学报》2018年第4期463-469,共7页Journal of China Pharmaceutical University

基　　金：江苏省自然科学基金资助项目(No.BK20161460);江苏高校优势学科建设工程资助项目~~

摘　　要：为考察青钱柳和桑叶组方的降血糖作用及安全性,以高热能饲料联合小剂量四氧嘧啶(alloxan,ALX)构建实验性糖尿病大鼠模型。各组大鼠分别给予蒸馏水(空白组、模型组)、二甲双胍(Met)、青钱柳水提物(CP)、桑叶水提物(ML)及不同比例的青钱柳和桑叶组方药物(CM1、CM2、CM3),检测血糖、血脂、胰岛素、糖耐量以及肝肾功能指标等,评价受试样品的降血糖效果及安全性。结果显示青桑组方药物组(CM1、CM2、CM3)大鼠的空腹血糖较模型组分别下降21.64%、16%、12.55%,其中CM1组空腹血糖、糖耐量以及胰腺组织形态显著改善,降血糖作用较优。桑叶水提物组(ML)及青桑组方药物组(CM3)与模型组相比,其TC、LDL-C指标显著下降(P<0.05),说明其有助于糖尿病大鼠血脂水平调控。各受试药物组对肝功能指标无不良影响,肾功能指标相较于模型组下降明显,说明其具有一定缓解肾损伤的作用。以上结果提示青桑组方药物可明显降低血糖,调节糖脂代谢,安全且无不良反应。The purpose of this study was to investigate the hypoglycemic effect and the safety of medicinal formula composed of Cyclocarya paliurus and Mulberry leaves.An experimental diabetic rat model was established by high energy diet plus small dosage of auoxan(ALX).At the same time,each group rats were given distilled water(blank and model),metformin(Met),Cyclocarya paliurus aqueous extract(CP),Mulberry leaves aqueous extract(ML)and the different proportions of aqueous extract mixtures of Cyclocarya paliurus and Mulberry leaves(CM1,CM2 and CM3),respectively.Fasting blood glucose(FBG),OGTT,TC,TG,LDL-C,HDL-C,insulin and liver and kidney function related index were gauged to evaluate the hypoglycemic effect and the safety of samples.The results showed that FBG level of the rats in CM1,CM2 and CM3 groups decreased 21.64%,16%and 12.55%,respectively,comparing with that of model group.Moreover,FBG,glucose tolerance and pancreatic tissue morphology were remarkably improved in CM1 group.TC and LDL-C levels of rats in ML and CM3 groups decreased significantly compared with the those of Model group(P<0.05),which showed ML and CM3 were beneficial to regulate the blood lipid level in diabetic rats.Furthermore,all the administration groups had no adverse effect on liver function index.The down regulation of kidney function index of CP,ML,CM1,CM2 and CM3 groups comparing with model group indicated that Cyclocarya paliurus and Mulberry leaves could alleviate the injury of liver and kidney.Our results demonstrated that the medicinal formula composed of Cyclocarya paliurus and Mulberry leaves were favorable to reduce blood glucose and can regulate lipid metabolism without liver and kidney toxicity.

关 键 词：青钱柳 桑叶 糖尿病 安全性 降血糖作用 

分 类 号：R965[医药卫生—药理学]