Rat model for study of the essence of spleen deficiency and dampness in Chinese medicine  

作　　者：Xiao-Chun Han Xu-Ming Ji Lin Liu Shuang Zhang Li Sun Qiu-Jian Feng Shi-Jun Wang 

机构地区：[1]College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan,China [2]Department of Special Examination,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan,China

出　　处：《Traditional Medicine Research》2018年第5期251-257,共7页TMR传统医学研究

基　　金：National Natural Science Foundation of China(NO.81703838);National Key Basic Research and Development Program(973 Program)funded project,spleen and dampness of traditional Chinese medicine drug research(NO.2013CB531803);Shandong Province,Chinese medicine science and technology development project,project number:2015-008;Shandong Province,Chinese medicine science and technology development project,project number:2017-027.

摘　　要：Objective:To explore the essence of spleen deficiency and dampness in Chinese medicine by successfully constructing a rat model with this syndrome.Methods:Rat models with the syndrome of dampness and spleen deficiency were established with the use of a high-fat,low-protein diet and excessive fatigue induced by loaded swimming.A total of 25 common clinical serological markers were tested.The T test,rank test,and partial least squares regression-discriminant analysis were used to analyze the data.Results:Total protein,albumin,motilin,interferon-γ,interleukin-2,immunoglobulin A,immunoglobulin G,and complement 3 levels in the model rats were lower than those in the control group(P=0.029,P=0.032,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,respectively).The serum creatinine,total cholesterol,aldosterone,antidiuretic hormone,gastrin,interleukin-4,somatostatin,atrial natriuretic peptide,and vasoactive intestinal peptide levels in the model group were higher than those in the control group(P<0.001,P=0.015,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,respectively).There was no statistical difference between the two groups in aspartate transaminase,alanine aminotransferase,globulin,albumin/globulin,blood urea nitrogen,high-density lipoprotein,and low-density lipoprotein.The model animal syndrome used two important variables in analysis with partial least squares regression-discriminant analysis.At the same time,the two dimensions were difficult to distinguish for each biological index.Conclusion:Spleen deficiency is often associated with dampness.The index that represents spleen deficiency can also represent dampness.Cytokines,immunoglobulins,and gastrointestinal hormones play a major causative role in both spleen deficiency and dampness.目的:通过构建脾虚水湿证型的动物模型探讨中医“脾虚”和“水湿”的本质。方法:以高脂低蛋白因素和游泳过度疲劳因素诱导脾虚水湿动物模型,选择临床常见的25项血清学指标进行检测,采用t检验、秩检验和回归分析对数据进行分析。结果:模型组大鼠的总蛋白、白蛋白、胃动素、干扰素-γ、白细胞介素-2、免疫球蛋白A、免疫球蛋白G、补体3水平均低于对照组(P=0.029,P=0.032,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001)。模型组的血清肌酐、总胆固醇、醛固酮、抗利尿激素、胃泌素、生长抑素、IL-4、心房钠尿肽和血管活性肠肽水平均高于对照组(P<0.001,P=0.015,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001,P<0.001)。两组在天冬氨酸转氨酶,丙氨酸转氨酶、球蛋白、白蛋白/球蛋白、血尿素氮、高密度脂蛋白,低密度脂蛋白水平无统计学差异。回归分析结果显示,模型组动物证候具有二维性,且此二维性在各生物学指标上难以明显分开。结论:脾虚常伴随水湿,细胞因子、免疫球蛋白、及胃肠激素是影响脾虚水湿的重要因素。

关 键 词：Spleen deficiency DAMPNESS Animal model 

分 类 号：R[医药卫生]