褪黑素对非生物胁迫下雨生红球藻中虾青素积累的影响  

THE EFFECTS OF MELATONIN ON THE ACCUMULATION OF ASTAXANTHIN IN HAEMATOCOCCUS PLUVIALIS LUGU UNDER ABIOTIC STRESS CONDITIONS

作　　者：岳陈陈 丁巍[1] 李涛[1] 赵鹏[1] 徐军伟[1] 余旭亚[1] YUE Chen-Chen;DING Wei;LI Tao;ZHAO Peng;XU Jun-Wei;YU Xu-Ya(Faculty of Life Sciences and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500

出　　处：《水生生物学报》2018年第5期1043-1049,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金地区科学基金项目(21766012和21666012)资助~~

摘　　要：以雨生红球藻Haematococcus pluvialis LUGU株为研究对象,研究在高光照和缺氮胁迫条件下,添加不同浓度褪黑素(melatonin,MLT)对雨生红球藻生长、虾青素积累、活性氧(ROS)、信号分子及dxs基因表达量的影响。结果表明,外源添加10μmol/L MLT可有效提高藻细胞中虾青素的含量,最高可达31.32 mg/g,是对照组(13.27 mg/g)的2.36倍;抑制了细胞内ROS水平,上调了信号分子一氧化氮(NO)和水杨酸(SA)的含量;此外,dxs基因表达水平比对照组明显提高,最高达11.3倍。研究表明,在非生物胁迫条件下,雨生红球藻中虾青素的大量积累可能与外源MLT调控细胞内ROS、信号分子及基因表达有关。In this research,the effects of different concentrations of melatonin(MLT)on the growth,accumulation of astaxanthin,reactive oxygen species(ROS),signal molecules,and the expression of DXS were studied in Haematococcus pluvialis LUGU under abiotic stress conditions(e.g.,high light,nitrogen starvation,etc.).The results showed that the astaxanthin content was efficiently enhanced in alga cells treated with 10μmol/L of MLT,the maximum astaxanthin content achieved was 31.32 mg/g,being 2.36 times higher than that of the control.In addition,MLT inhibited intracellular ROS levels and up-regulated the content of signaling molecules(nitric oxide and salicylic acid).Additionally,the relative expression level of DXS gene was increased by 11.3-fold higher than that of control.These results demonstrated that the massive accumulation of astaxanthin in Haematococcus pluvialis is associated with the regulation of intracellular ROS,signaling molecules,and gene expression by exogenous MLT under abiotic stress conditions.

关 键 词：褪黑素 雨生红球藻 虾青素 活性氧 信号分子 

分 类 号：Q945.1[生物学—植物学]