P130CAS对食管癌细胞ECA109中Angiopoietin-2表达及其生物学行为的影响  

Effect of P130CAS on Angiopoietin-2 expression in esophageal cancer ECA109 cells and its biological behaviors

作　　者：唐绪容[1] 黄伟 屈虹[1] 马洪飚 罗锋 TANG Xu-rong;HUANG Wei;QU Hong;MA Hong-biao;LUO Feng(Operation Room,Children’s Hospital of Chongqing Medical University/Chongqing Key Laboratory of Pediatrics,Chongqing 400014,China;Department of Thoracic Surgery,Chongqing General Hospital,Chongqing 400013,China)

机构地区：[1]重庆医科大学附属儿童医院手术室/儿科学重庆市重点实验室,重庆400014 [2]重庆市人民医院胸外科,重庆400013

出　　处：《临床与实验病理学杂志》2018年第8期840-844,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：重庆市渝中区科技计划项目(20150128)

摘　　要：目的探讨抑制P130CAS(P130 Crk-associated substrate)基因对食管癌细胞ECA109中Angiopoietin-2表达和生物学行为的影响。方法构建P130CAS基因siRNA慢病毒载体,感染ECA109细胞,荧光显微镜观察感染效率,应用RT-PCR和Western blot法检测其干扰效率。应用CCK-8法、划痕实验、Transwell实验、Western blot法检测抑制P130CAS基因表达对ECA109细胞增殖、迁移、侵袭能力和对Angiopoietin-2蛋白表达的影响。结果成功构建PLVT716 siRNA慢病毒载体,ECA109细胞P130CAS mRNA和蛋白表达明显下调(P<0.05),并建立稳定细胞株;抑制P130CAS基因表达导致ECA109细胞增殖、迁移和侵袭能力显著减弱(P<0.05),同时Angiopoietin-2表达明显下调(P<0.05)。结论抑制P130CAS基因表达可抑制食管癌ECA109细胞的增殖、迁移和侵袭,同时可下调Angiopoietin-2表达。Purpose To discuss the effect P130CAS gene knockdown on Angiopoietin-2 expression in esophageal cancer ECA109 cells and its biological behaviors.Methods The siRNA lentiviral vectors of P130CAS gene were constructed,and then transfect into ECA109 cells.The infection efficacy of ECA109 cells was observed by fluorescent microscopy.The interfering effect of P130CAS gene in the ECA109 cells was measured by RT-PCR and Western blot.The viability,migration,invasion and Angiopoietin-2 expression of ECA109 cells were measured by CCK-8,Transwell experiment,scratch adhesion test and Western blot,respectively.Results The PLVT716-siRNA lentiviral vector was successfully constructed.The mRNA and protein of P130CAS gene in ECA109 cells were down-regulated(P<0.05),which were used to construct stable cell strain.After down-regulated P130CAS expression,viability,migration and invasion of ECA109 cells were significantly decreased(P<0.05),and its Angiopoietin-2 expression was inhibited(P<0.05).Conclusion P130CAS expression knockdown can decrease viability,migration,invasion and Angiopoietin-2 expression of ECA109 cells.

关 键 词：食管肿瘤 P130CAS ANGIOPOIETIN-2 慢病毒 

分 类 号：R735.1[医药卫生—肿瘤]