小麦CBL4基因密码子使用特征分析  

Analysis of Codon Bias of CBL4 Gene in Triticum aestivum

作　　者：陈文烨 焦义然 杨军峰 刘永伟[2] 杨帆[2] 董福双[2] 王海波[2] 杜进民[1] 周硕[2] CHEN Wenye;JIAO Yiran;YANG Junfeng;LIU Yongwei;YANG Fan;DONG Fushuang;WANG Haibo;DU Jinmin;ZHOU Shuo(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050000,China;Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,China;Hebei Wangfeng Seed Industry Co.,Ltd.,Xingtai 054900,China)

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050000 [2]河北省农林科学院遗传生理研究所/河北省植物转基因中心,河北石家庄050051 [3]河北旺丰种业有限公司,河北邢台054900

出　　处：《河南农业科学》2018年第8期11-16,共6页Journal of Henan Agricultural Sciences

基　　金：国家重点研发计划"七大作物育种"重点专项(2016YFD0101802);国家自然科学基金青年科学基金项目(31600216);河北省自然科学基金青年科学基金项目(C2017301066);河北省现代农业创新工程项目(494-0402-YBN-RDC4)

摘　　要：采用Codon W和Usage Codon软件分析小麦CBL4基因密码子偏好性,并将其与其他17种不同目植物CBL4基因密码子偏好性进行比较,利用最小进化法分析多种植物的亲缘关系,以期为小麦CBL4基因改良以提高对干旱、低温和盐胁迫等逆境的抗性提供理论依据。结果表明,GC含量对小麦CBL4基因密码子偏好性具有重要的影响,小麦的G+C所占比例达58.0%,其中以第3位GC含量为主,而马铃薯对密码子的使用特征与小麦差异最大,形成鲜明的对比。小麦偏好使用CUC、AGG、UAA、AUC、GUG、GCG、ACG、UGC和GGC密码子,小麦等植物密码子偏好性结果与系统发育分析得出的亲缘关系结果一致。CodonW and Usage Codon softwares were used to analyze the codon bias of wheat,the codon bias of other 17 plant species were compared with that of wheat,and the genetic relationships of 18 plant species were analyzed by the minimum evolution method,so as to provide theoretical basis for improvement of resistance to drought,cold,salt through improvement of CBL 4 gene of wheat.The results showed that GC content had important influence on the codon bias of CBL 4 gene in wheat,GC content was 58.0%in wheat,GC content of the third codon position was main.The difference between the use of the codon in Solanum tuberosum and Triticum aestivum was the greatest.The wheat had bias for CUC,AGG,UAA,AUC,GUG,GCG,ACG,UGC and GGC.The result of phylogenetic relationship of multiple plants was consistent with the result of codon bias.

关 键 词：小麦 CBL4 相对同义密码子使用度 密码子偏好性 

分 类 号：S512.1[农业科学—作物学]