鸟嘌呤和异亮氨酸促进丁酸梭菌生长  

Guanine and Ile promote the cell growth of Clostridium butyricum

作　　者：刘颖[1] 杨华[1] 宋昭 陈雄[1] 黄亚男 王志[1] LIU Ying;YANG Hua;SONG Zhao;CHEN Xiong;HUANG Ya-nan;WANG Zhi(Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Collaborative Innovation Center for IndustrialFermentation,Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology,Wuhan 430068,China;Henan Nanjiecun(Group)Co.Ltd.,Linying 462600,China)

机构地区：[1]湖北工业大学,发酵工程教育部重点实验室,湖北省工业发酵协同创新中心,湖北省工业微生物重点实验室,武汉430068 [2]河南省南街村(集团)有限公司,河南临颍462600

出　　处：《食品与发酵工业》2018年第8期34-40,共7页Food and Fermentation Industries

基　　金：湖北省自然科学基金(2014CFB604)。

摘　　要：为了延缓丁酸梭菌芽孢形成,提高丁酸梭菌的生长效率,在20 L发酵罐水平研究了发酵基料添加鸟嘌呤(0.2 g/L)、Ile(0.5 g/L)对丁酸梭菌生长、酸代谢的影响。结果表明:添加鸟嘌呤或Ile的最大生物量分别达到5.31×10~8CFU/mL和4.65×10~8CFU/mL,较对照(4.32×10~8CFU/mL)分别提高了22.91%和7.64%;丁酸的菌体得率(Yp/x)分别较对照(0.37 g/g)减少了48.64%和40.54%。鸟嘌呤和Ile均显著降低了丁酸的生成效率、促进了细胞的生长、延缓了芽孢的形成。当发酵基料同时添加鸟嘌呤和Ile时,生物量达到5.4×10~8CFU/mL,比对照提高了25%,为丁酸梭菌的工业化生产提供了新思路。In order to delay the sporulation and improve the growth efficiency of Clostridium butyricum,the effects of guanine(0.2 g/L)and Ile(0.5 g/L)on the growth and acids metabolism of C.butyricum in 20 L bioreactor were investigated.The results showed that the maximum biomass with the addition of guanine or Ile reached 5.31×10 8 CFU/mL and 4.65×10 8 CFU/mL,respectively,which was increased by 22.91%and 7.64%compared with the control(4.32×10 8 CFU/mL).The coefficient of butyric acid to maximum biomass(Y p/x)was decreased by 48.64%and 40.54%compared with the control(0.37 g/g),respectively.Gunanine and/or Ile addition could reduce the specific butyrate productivity,enhance the growth efficiency and delay the sporulation of C.butyricum.The biomass reached 5.4×10 8 CFU/mL when guanine(0.2 g/L)and Ile(0.5 g/L)were simultaneously added to the fermentation medium,which was 25%higher than that of the control.This research provides new methods for industrial fermentation of C.butyricum.

关 键 词：丁酸梭菌 鸟嘌呤 异亮氨酸 芽孢 

分 类 号：TQ921.4[轻工技术与工程—发酵工程]