高产舍雷肽酶的粘质沙雷氏菌诱变选育  

Mutation breeding of Serratia marcescens strains for serrapeptase high-yield production

作　　者：梅建凤[1] 蔡少芬 李靓 易喻[1] 应国清[1] MEI Jian-feng;CAI Shao-fen;LI Liang;Yi Yu;YING Guo-qing(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China;Zhejiang Tianke High Technology Development Co.Ltd.,Hangzhou 310012,China)

机构地区：[1]浙江工业大学药学院,浙江杭州310014 [2]浙江天科高新技术发展有限公司,浙江杭州310012

出　　处：《食品与发酵工业》2018年第8期177-180,共4页Food and Fermentation Industries

摘　　要：舍雷肽酶是一种来自粘质沙雷氏菌(Serratia marcescens)的碱性蛋白酶,具有良好的抗炎和抗肿胀作用,应用于临床疾病的治疗已有多年。为了获得产酶活力高、传代稳定且不产灵菌红素的舍雷肽酶发酵生产菌株,以1株分离自家蚕(Bombyx mori)肠道,产舍雷肽酶的粘质沙雷氏菌LL-413为出发菌株,用紫外线和微波连续诱变,经过筛选,获得了2株产酶能力高,不产灵菌红素,且传代稳定的突变株MW-9和MW-14菌株,产酶活力分别达到982 U/mL和956 U/mL,是原始菌株的1.98倍和1.92倍。Serrapeptase is a kind of alkaline protease from Serratia marcescens.Due to its excellent anti-inflammatory and anti-swelling function,serrapeptase has been applied to treat some diseases in clinical for many years.In order to obtain strains with the characteristics of fine hereditary stability and prodigiosin negative for high-yield production of serrapeptase,a strain isolated from the intestine of silkworm(Bombyx mori),S.marcescens LL-413 was mutated by ultraviolet and microwave continuously.As results,2 strains,designated as MW-9 and MW-14,were selected out.Their productivity of serrapeptase was 982 U/mL and 956 U/mL,which respectively was 1.98 and 1.92 times higher than that of the original strain.

关 键 词：舍雷肽酶 粘质沙雷氏菌 诱变育种 紫外线 微波 

分 类 号：TQ925[轻工技术与工程—发酵工程]