Box-Behnken设计优化山楂叶总黄酮提取纯化工艺及其抑菌活性研究  

Optimization of Extraction and Purification Process on Total Flavone of Hawthorn Leaves by Using Box-Behnken Design and Analysis of Its Bacteriostasis

作　　者：宋小锋 原增艳 李鹏旭 许平辉 刘勇 SONG Xiaofeng;YUAN Zengyan;LI Pengxu;XU Pinghui;LIU Yong(Sanquan College of Xinxiang Medical University,Xinxiang 453000,Henan China;Xinxiang Biocaro Co.Ltd.,Xinxiang 453000,Henan China;Henan Guangkang Pharmaceutical Co.Ltd.,Anyang 456100,Henan China)

机构地区：[1]新乡医学院三全学院,河南新乡453000 [2]新乡博凯生物技术有限公司,河南新乡453000 [3]河南省广康药业有限公司,河南安阳456100

出　　处：《河南科学》2018年第8期1215-1220,共6页Henan Science

基　　金：河南省科技攻关项目(172102310311);新乡医学院三全学院创新团队(STD201603)

摘　　要：利用Box-Behnken设计优化山楂叶总黄酮的提取及纯化工艺,优化实验表明,提取工艺参数:提取剂乙醇体积分数65%、提取温度(67±1)℃、提取时间80 min,山楂叶总黄酮提取率可达92.2%;纯化工艺参数:洗脱剂乙醇体积分数60%、洗脱剂用量4.4倍柱床体积(BV)、洗脱流速3.7 BV/h,提取物中山楂叶总黄酮平均质量分数可达86.8%.此为最佳提取工艺和最佳纯化工艺参数.体外抑菌试验表明,山楂叶提取物对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌均具有良好的抑菌功效.We optimize the extraction and purification process of Total Flavone of Hawthorn Leaves which is used by Box-Behnken.The experiment shows that the optimum extraction conditions of TFHL are 65%ethanol as solvent at(67±1)℃for 80 min and the extraction rate of TFHL is 92.2%.The optimum purification process of TFHL are purified with 4.4 BV of 60%ethanol as the eluent,and the flow rate is 3.8 BV/h.Under these conditions,the purification of TFHL is 86.8%.The bacteriostasis function test shows that the hawthorn leave extract has the obvious inhibiting effect on S.aureus and MRSA.

关 键 词：山楂叶总黄酮 山楂叶 Box-Behnken 抑菌活性 

分 类 号：Q819[生物学—生物工程]