云南宣威地区肺癌长链非编码RNA、mRNA基因表达谱检测与竞争性内源RNA分析  

Expression profile of lncRNA and mRNA and analysis of ceRNA in lung cancer patients in Xuanwei

作　　者：胡家伦[1] 王玉明[1] 陈冉[1] 肖成[1] 郎昆 柳杰 HU Jia-lun;WANG Yu-ming;CHEN Ran;XIAO Cheng;LANG Kun;LIU Jie(Department of Clinical Laboratory,the Second Affiliated Hospital,Kunming Medical University,Kunming 650000,Yunnan,China)

机构地区：[1]昆明医科大学第二附属医院检验科,昆明医学硕士研究生650000

出　　处：《医学研究生学报》2018年第8期818-823,共6页Journal of Medical Postgraduates

基　　金：云南省科技厅昆医联合专项应用基础研究基金(2015FB047)

摘　　要：目的云南宣威地区是中国乃至世界肺癌高发区,探究长链非编码RNA(lncRNA)和mRNA的差异表达,构建竞争性内源RNA(ceRNA)共表达网络,为宣威地区肺癌的分子诊断和治疗提供新思路。方法采用晶芯lncRNA+mRNA表达谱芯片对10对宣威地区肺癌组织及癌旁正常组织进行检测,用生物信息学的方法(GO,Pathway和networkanalysis)对差异表达的mRNAs进行分析。结果通过肺癌标本与癌旁正常组织对比,从中找到差异表达的lncRNA有384条(差异倍数≥2.0,P<0.05),其中表达上调的有78条,表达下调的有306条。上调表达差异最大的是ab Parts(差异倍数:7.522),下调表达差异最大的是XLOC_012046(差异倍数:9.001)。946条差异表达的mRNA(差异倍数≥2.0,P<0.05),其中上调和下调最具意义的是SPP1(差异倍数:16.500)和AGER(差异倍数:14.377)。lncRNA和mRNA在宣威地区肺癌中呈现出高低表达的两种趋势,差异表达的mRNA在生物过程、分子功能、细胞组成中分别以单一生物过程、结合和膜功能方面的数目差异最明显。上调表达的mRNA主要参与的信号通路有钙离子信号通路、吞噬体、造血细胞谱系等,下调表达差异的mRNA主要参与了造血细胞谱系、钙离子信号通路等方面的通路。lncRNA和mRNA之间呈一对一或一对多的相关,其中p14245(LINC00472)与Q9H8W2和XLOC_005764共表达。ENSG00000244513.2(ceRNA-score:0.461)和ENSG00000257424.1(ceRNA-score:0.372)所调节的SEMA5A和SEMA6A在mRNA表达谱中都呈下调表达。SEMA5A主要参与细胞黏附、生物黏附、脉管系统发展等功能,SEMA6A主要参与细胞表面受体连锁信号转导、细胞运动、细胞骨架组织等功能。结论通过微阵列找到宣威地区肺癌组织中差异表达的lncRNA表达谱可能会成为宣威地区肺癌的新型生物标志物或治疗靶点。Objective Xuanwei,Yunnan,is one of the areas with the highest incidence of lung cancer in China and in the world as well.This study was to explore the differential expressions of lncRNAs and mRNAs,establish a competing endogenous RNA(ceRNA)coexpression network,and find some new ideas for the diagnosis and treatment of lung cancer in the Xuanwei area. Methods Using the gene chip of the lncRNA+mRNA expression profile,we examined 10 pairs of samples of lung cancer and adjacent normal tissues from the patients in the Xuanwei area and analyzed differentially expressed mRNAs with such bioinformatics methods as gene ontology(GO),Kyoto Encyclopedia Genomes(KEGG)pathway and network analyses. Results By comparison between the lung cancer and adjacent normal tissues,384 differentially expressed lncRNAs were identified(fold change≥2.0,P<0.05),in which 78 were upregulated,with abParts as the most differentially expressed(fold change=7.522,and 306 downregulated,with XLOG-012046 as the most differentially expressed(fold change=9.001).Among the 946 differentially expressed mRNAs identified(fold change≥2.0,P<0.05),SPP1 was the most significantly upregulated(fold change=16.500)and AGER was the most significantly downregulated(fold change=14.377).Both up-and downregulated expressions of lncRNA and mRNA were found in the lung cancer tissue of the patients,the upregulated expression of mRNA involved mainly the calcium ion signaling pathway,phagosomes and hematopoietic cell lineage,while the downregulated expression of mRNA chiefly involved in the hematopoietic cell lineage and calcium ion signaling pathway.lncRNAs were correlated with mRNAs in a one-to-one or one-to-many manner,with the coexpression of p14245(LINC00472)with Q9H8W2 and XLOG-005764.The expressions of SEMA5A and SEMA6A regulated by ENSG00000244513.2(ceRNA score=0.461)and ENSG00000257424.1(ceRNA score=0.372)were both decreased in the mRNA expression profile.SEMA5A participated mainly in cell adhesion,bioadhesion,and vasculature development,while SEMA6A chiefly in cel

关 键 词：宣威地区 肺癌 长链非编码RNA 竞争性内源RNA 基因芯片 

分 类 号：R734.2[医药卫生—肿瘤]