薤白多糖的提取及体外抗氧化活性研究  

Extraction of polysaccharide from Allium macrostemon Bunge and their antioxidant activity in vitro

作　　者：韩秋菊[1] 王晓玲[1] 王菲[1] 齐佳慧[1] 李洪影 冉令辉 王战勇[1] HAN Qiu-ju;WANG Xiao-ling;WANG Fei;QI Jia-hui;LI Hong-ying;RAN Ling-hui;WANG Zhan-yong(School of Chemistry,Chemical Engineering and Environmental Engineering, Liaoning Shihua University,Fushun 113001,China)

机构地区：[1]辽宁石油化工大学化学化工与环境学部,辽宁抚顺113001

出　　处：《应用化工》2018年第8期1680-1683,共4页Applied Chemical Industry

基　　金：辽宁省高等学校杰出青年学者成长计划(LJQ2014040)

摘　　要：用热水浸提法提取薤白中的生物多糖,采用响应面法对提取条件进行优化,并研究了薤白多糖的体外抗氧化活性。结果表明,最佳提取条件为:提取温度72℃,提取时间1.5 h,液料比20∶1 m L/g,薤白多糖提取率(5.26±0.014)%。薤白多糖具有一定的体外抗氧化能力,但活性低于同浓度的Vc,其中薤白多糖对羟基自由基的清除能力相对较强,IC50值为1 591.7μg/m L;对超氧阴离子自由基的清除能力及对Fe3+的还原能力较弱。The technology of polysaccharides extraction from Allium macrostemon Bunge was studied by hot water extraction method and the extraction conditions were optimized by response surface methodology.The antioxidant activity in vitro was evaluated by artificial free radicals in detail.The results showed that the optimum extraction conditions were:extraction temperature 72℃,extraction time 1.5 h,ratio of liquid to material 20∶1 mL/g.Under the optimum conditions,the extraction ratio was(5.26±0.014)%.The Allium macrostemon Bunge polysaccharide had certain antioxidant capacity in vitro but less than Vc.The Allium macrostemon Bunge polysaccharide had better scavenging effect on hydroxyl radical with IC 50 of 1 591.7μg/mL and had lower scavenging effect on superoxide anion radical and weak reductive effect on Fe^3+.

关 键 词：薤白 多糖 提取 抗氧化活性 

分 类 号：TQ911[化学工程] TS255[轻工技术与工程—农产品加工及贮藏工程]