Folin-Ciocalteu比色法测定牡丹籽饼、牡丹叶、牡丹籽种壳中总多酚的含量  

Determination of total polyphenols in peony seed cake,peony leaf and peony shell by Folin-Ciocalteu colorimetric method

作　　者：王海坤 张存劳 陈程[2] WANG Hai-kun;ZHANG Cun-lao;CHEN Cheng(Shaanxi Xingsenyuan Bioscience Inc,Xianyang 712035,China;Pharmaceutical Institute,Xi’an Medical University,Xi’an 710021,China)

机构地区：[1]陕西兴森源生物科技有限公司,陕西咸阳712035 [2]西安医学院药学院,陕西西安710021

出　　处：《应用化工》2018年第8期1794-1796,1802,共4页Applied Chemical Industry

基　　金：陕西省教育厅2017自然科技专项(17JK0649)

摘　　要：采用Folin-Ciocalteu比色法测定牡丹籽饼、牡丹叶、牡丹籽种壳中总多酚含量,以没食子酸为对照品,通过单因素实验对测定条件进行优化。结果表明,Folin-Ciocalte比色法测定牡丹籽饼、牡丹叶、牡丹籽种壳中总多酚含量的最优条件为:福林酚试剂2.5 m L,10%Na_2CO_3溶液2.5 m L,在30℃条件下避光30 min,测定其在770 nm处吸光度,没食子酸在2.018~10.09μg/m L范围内与吸光度值线性关系良好,回归方程为A=0.667C-0.014 9(r=0.999 6),精密度、稳定性、重复性、加样回收率的相对标准差(RSD)分别为0.15%,1.87%,2.23%,2.44%。牡丹籽饼、牡丹叶、牡丹籽种壳中总多酚含量依次为6.35,9.16,1.60 mg/g。该方法简便、可行、重复性好,可用于牡丹籽饼、叶、荚中总多酚的定量分析。To determine the total polyphenols content in peony seed cake,peony leaf and peony shell by Folin-Ciocalteu colorimetric method.The optimum conditions were investigated using gallic acid by single experiments.The results showed that by applying Folin-Ciocalteu reagent 2.5 mL and 2.5 mL 10%Na 2CO 3 at 30℃for reaction in 30 min by 770 nm.The regression and absorbance valte had a good linear relationship when the gallic acid was in range of 2.018~10.09μg/mL.The regression equation was as follow:A=0.667C-0.014 9(r=0.999 6),the precision,stability,repeatability and average recovery rate were 0.15%,1.87%,2.23%and 2.44%.The total polyphenols content of peony seed cake,peony leaf and peony shell were 6.35 mg/g,9.16 mg/g and 1.60 mg/g.The method had merits of simple,feasible and good reproducibility,which is suitable for analysis the total polyphenols in peony seed cake,peony leaf and peony shell.

关 键 词：牡丹籽饼 牡丹叶 牡丹籽种壳 总多酚 定量分析 Folin-Ciocalteu比色法 

分 类 号：TQ469[化学工程—制药化工] R283.62[医药卫生—中药学]