S2B-5 Rapid Release of GABA Inhibiting Epilepsy in Vivo  

作　　者：DING Ran LI Yuan-yuan QIAO Zhi-hong LIU Nan-nan ZHANG Xin WEI Liang-peng HOU Meng-zhu LIU Ai-li ZHENG Chen FU Bing XU Ying-shun MA Zheng-guan HOU Shao-wei WANG Fei-fei YUAN Shi-yang LIU Shu-jing ZHANG Yan SHEN Hui 

机构地区：[1]Tianjin Medical University [2]Zhenyin Medical University

出　　处：《神经药理学报》2018年第4期48-49,共2页Acta Neuropharmacologica

摘　　要：Objective:Epilepsy is a multi-etiological brain dysfunction syndrome characterized by synchronously repeated spontaneous discharges from neuronal cells.Its pathogenesis involves excitatory/inhibitory imbalance,ion channel abnormalities and may be related to the abnormal structure and function of neurotransmitter receptors.The research was to observe the effects of rapid increase inhbitory neurotransmitter GABA on the excitatory/inhibitory imbalance,ion channel abnormalities and abnormal neurotransmitter receptors in living epileptic mice from the level of free moving animal,cell,sub-cell and receptor.Methods:The techniques of fiber photometry and laser uncage of Rub-GABA for epileptic free moving mice induced by administration of Kainic acid(KA)or 4-AP,local field potentials(LFP),multicell bolus lording of calcium(OGB-1AM),virus transfection of calcium-sensitive protein GCaMP6f,transfection of fluorescent labeled AMPA receptors in cortical neurons by in utero electroporation,two-photon uncage of Rubi-GABA,two-photon imaging,two-photon calcium imaging,twophoton cell attach,two-photon targeted patch clamp and two-photon shadow patch clamp in living epileptic mice induced by 4-AP were used.Results:Laser photolysis of Rubi-GABA in hippocampal CA1 could immediately alleviate KA induced acute epileptic seizures in living mice.In cellular level,two-photon uncage Rub-GABA or rapid release of GABA significantly decreased the number of neurons releasing the calcium signals by multicell bolus lording of calcium,inhibited spikes recording by LFP and immediately inhibited both calcium signals and spikes using two-photon cell attaching technique in the living epileptic mice induced by 4-AP.Two-photon uncage Rub-GABA significantly decreased spikes induced by 4-AP recording with two-photon shadow patch clamp in vivo.In sub-cellular level,two-photon Rubi-GABA uncage of dendritic spines of cortical neurons transfected with GCaMP6f obviously decreased the frequency and amplitude of calcium signal on those dendritic spines in 4-AP i

关 键 词：OGB-1AM labeled AMPA RECEPTORS 

分 类 号：R73[医药卫生—肿瘤]