X线相衬CT成像显示人体胆道闭锁样品微脉管结构  

Microvasculature imaging of biliary atresia samples based on X-ray phase-contrast CT

作　　者：孙梦燏 吕文娟 赵新颜[2] 秦莉莉 胡春红 SUN Mengyu;LYU Wenjuan;ZHAO Xinyan;QIN Lili;HU Chunhong(College of Biomedical Engineering,Tianjin Medical University,Tianjin 300070,China;Liver Research Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]天津医科大学生物医学工程与技术学院,天津300070 [2]首都医科大学附属北京友谊医院肝病中心,北京100050

出　　处：《中国医学影像技术》2018年第9期1318-1322,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(81671683;81670545;81371549);天津市自然科学基金(16JCYBJC28600)

摘　　要：目的探讨X射线相衬CT(PCCT)对人体胆道闭锁(BA)样品中脉管显微结构的显示作用。方法将4份人体BA样品进行冲洗、固定并以乙醇脱水干燥,采用上海同步辐射光源BL13W1线站进行PCCT成像,通过图像处理分别获得滤波反投影算法重建图像、样品整体及微脉管的3D重建图像,并对不同类型脉管进行区分。而后对样品进行石蜡包埋,4μm切片,并行平滑肌细胞抗体(SMA)、细胞角蛋白19抗体(CK19)免疫组化染色及苦味酸天狼星红染色组织病理检查。对比观察滤波反投影算法及微脉管3D重建图像与相应病理图。结果滤波反投影算法重建CT图像及微脉管3D重建图像均可用以区分人体BA样品中的肝动脉、胆管及增生胆管、门静脉,并清晰显示不同类型脉管的结构特征,所示微脉管结构与组织病理学检查相符。结论结合滤波反投影重建算法及3D可视化技术,PCCT能够清晰显示离体BA组织的微脉管结构。Objective To explore the application value of X-ray phase-contrast CT(PCCT)in investigation of the microvasculature of human biliary atresia(BA)tissues.Methods Four resected samples of BA children were douched and fixed.After alcohol dehydration,the samples were examined with Shanghai Synchrotron Radiation Facility(SSRF)beamline BL13W1 PCCT.The images were reconstructed using filtered back projection(FBP)algorithm.Combined with 3D visualization technology,the whole structure and microvasculature of the samples were revealed and used for differentiating different vessels.Then the samples were embedded in paraffin,cut into 4μm sections and stained with Sirius red,cytokeratin 19(CK19)and stone mastic asphalt(SMA).The reconstruction images of FBP algorithm and the 3D microvascular images were observed and compared with the corresponding histological findings.Results Both of the reconstruction images of FBP algorithm and 3D microvascular images could be used for differentiation of different types of vessels,including hepatic artery,bile duct and neoformative bile duct,as well as portal vein.The microstructure characteristics of different types of vessels in BA samples were shown accurately and were consistent with histology.Conclusion Combined FBP algorithm with 3D visualization technique,PCCT can be used to clearly display the microstructures of BA tissues in vitro.

关 键 词：X射线相衬成像 体层摄影术 X线计算机 胆道闭锁 微脉管 3D可视化 

分 类 号：R-332[医药卫生] R814.42