5-氮杂胞苷对肺癌细胞株功能影响及其机制  

The effects of 5-azocytoside on the function of lung cancer cell lines and related mechanisms

作　　者：许银辉 赖麒 张小影 陈强 唐亮 刘栋 杜振宗 XU Yinhui;LAI Qi;ZHANG Xiaoying;CHEN Qiang;TANG Liang;LIU Dong;DU Zhenzong(Department of Cardiothoracic Surgery,the 2nd Affiliated Hospital of Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院第二附属医院胸心外科,广西桂林541199

出　　处：《实用医学杂志》2018年第17期2839-2843,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81160296);广西自然科学基金项目(编号:2013GXNSFCB019005)

摘　　要：目的探讨A549和H358细胞株经表观治疗后,对其生长、侵袭和凋亡的影响,分析可能的作用机制。方法 5-氮杂胞苷处理A549和H358细胞株24和48 h后,运用流式细胞仪检测细胞增殖,Transwell方法检测细胞侵袭能力,赫斯特染色荧光显微镜观察细胞凋亡;RT-PCR和Western blot检测处理后DNMT1、RASSFA和APC基因在肺癌、BEAS-2B细胞株中的m RNA转录和蛋白表达。结果两细胞株中DNMT1在mRNA转录和蛋白表达水平较BEAS-2B细胞株高;治疗后,RASSFA和APC基因活性增加,DN-MT1基因活性则受抑制,A549和H358细胞株的生长和侵袭能力减弱,凋亡增加,且与处理时间呈正比关系。结论 5-氮杂胞苷可以通过抑制DNMT1活性而使肺癌细胞功能减弱甚至丧失,RASSFA和APC基因去甲基化可能参与其中。Objective To investigate the effect of 5-azacytidine on growth,invasion and apoptosis of A549 and H358 cell lines,and to reveal the possible mechanism.Methods A549 and H358 cell lines were treated with 5-azacytidine for 24 and 48 h,respectively.Flow cytometry was performed to detect cell proliferation.Transwell migration method was used to detect cell invasion.Hearst staining was used to detect apoptosis by fluorescence microscopy.The mRNA and protein expression of DNMT1,RASSFA and APC genes in lung cancer and BEAS-2B cell lines were determined by RT-PCR and Western blot,respectively.Results The mRNA and protein expression of DNMT1 were higher in A549 and H358 cell lines than those in the BEAS-2B cell line.After 5-azacytidine treatment,the RASSFA and APC gene activity was increased,the DNMT1 gene activity was inhibited,and the growth and invasion ability of the A549 and H358 cell lines were weakened,with the increased apoptosis in a time-dependent manner.Conclusion 5-Azacytidine can weaken or even impair the function of lung cancer cells by inhibiting the activity of DNMT1,as well as the demethylation of RASSF1A and APC.

关 键 词：肺癌细胞株 表观治疗 DNMT1 RASSFA APC 

分 类 号：R734.2[医药卫生—肿瘤]