玉米泛素结合酶基因家族的生物信息学及表达分析  

Bioinformatics and expression analysis of ubiquitin conjugating enzyme gene family in maize

作　　者：陈曙[1] 赵秋芳[1] 陈宏良[1] 贾利强[1] CHEN Shu;ZHAO Qiu-fang;CHEN Hong-liang;JIA Li-qiang(Institute of South Subtropical Corp Research,Chinese Academy of Tropical Agricultural Science/Key Laboratory of Tropic Fruit Biology,Ministry of Agriculture and Rural Affairs,Zhanjiang,Guangdong 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室,广东湛江524091

出　　处：《南方农业学报》2018年第8期1476-1483,共8页Journal of Southern Agriculture

基　　金：中央级非盈利性科研院所基本科研业务费项目(1630062017015);农业部国际交流与合作项目(13232697606221573)

摘　　要：【目的】利用生物信息学分析玉米泛素结合酶(E2)基因家族的理化性质和功能,并研究低磷处理下泛素E2基因在玉米幼苗不同组织部位的表达情况,为深入研究泛素E2基因响应低磷胁迫的分子机制提供理论参考。【方法】以MEGA 6.0邻接法(NJ)构建拟南芥、水稻和玉米E2基因家族成员的系统发育进化树,利用生物信息学方法对75个玉米E2基因进行序列分析及理化性质预测。对玉米幼苗进行低磷处理,分别在0、1、6和24 h时采集其根部和叶片样品,并分别通过实时荧光定量PCR(qRT-PCR)检测其E2亚家族XVIII成员基因即ZmUBC17、ZmUBC18、ZmUBC48、ZmUBC57和ZmUBC58在玉米幼苗不同组织中的表达情况。【结果】拟南芥、水稻和玉米的E2基因可划分为18个亚家族,各亚家族间基因功能和进化关系较相近,但含有的E2基因数量差异明显,以亚家族VI的成员最多,亚家族X、XII和XV的成员数最少,均为3个,各亚家族中均分别含拟南芥、水稻和玉米E2基因各1个。玉米E2基因的编码区序列(CDS)长度为402~2616 bp,编码133~871个氨基酸,外显子数目数量存在明显差异,以4~6个居多,最少为1个,最多为12个。86.8%的玉米E2蛋白为不稳定蛋白。玉米E2亚家族XVIII成员中,ZmUBC18、ZmUBC48、ZmUBC57和ZmUBC58蛋白UBC结构域序列具有高度相似性,但与ZmUBC17存在明显差异,且这4个基因在玉米根和叶中均有表达,基因表达模式相似,整体上在低磷处理6 h时的表达量最高;低磷处理下ZmUBC17基因在根中不同时间点的表达量变幅较小,但在叶0~24 h时的表达量呈逐渐升高的变化趋势,尤其是24 h时表达量达最高,表明ZmUBC17基因的响应部位是叶片。【结论】玉米泛素E2蛋白可能参与调控对磷元素的吸收,尤其是ZmUBC17基因具有组织表达特异性,在叶片中大量表达,推测ZmUBC17基因通过调控玉米对磷元素吸收和转运间接影响光合作用效率。【Objective】The ubiquitin conjugating enzyme(E2)gene family members were searched by using bioinformatics tools to analyze the physicochemical property,for studying how E2 genes expressed in maize seedlings when dealing with low phosphorous.This would lay a foundation for further study on the molecular mechanism of ubiquitin E2 genes responsing to low phosphorus stress.【Method】A phylogenetic tree was constructed using MEGA 6.0 neighborjoining(NJ)method based on arabidopsis,rice and maize E2 gene family members.The prediction of physiological and chemical properties and sequencing analysis of 75 maize E2 genes were performed using bioinformatics method.Maize seedlings were treated with low phosphorus,and root samples and leaf samples were collected at 0,1,6 and 24 h.The real-time fluorescent quantitative PCR(qRT-PCR)was performed to test the expression of E2 subfamily XVIII members(ZmUBC17,ZmUBC18,ZmUBC48,ZmUBC57,ZmUBC58)in different parts responding to low phosphorus at 0,1,6 and 24 h in maize.【Result】The arabidopsis,rice and maize E2 gene family members were divided into 18 subfamilies.A similar function and the close evolutionary relationship were found in different subfamilies.The E2 genes number contained varied largely.Subfamily VI had the largest members,while X,XII and XV had the least(three members),X,XII and XV contained one maize gene,one arabidopsis gene and one rice gene.The results showed that the coding region sequence(CDS)length ranged from 402 to 2616 bp,encoded amino acids were between 133 to 871.The numbers of E2 exon varied from 1 to 12,while the most number existed between 4 to 6.The difference was large.86.8%maize E2 proteins were authenticated unsteadily.ZmUBC17,ZmUBC18,ZmUBC48,ZmUBC57 and ZmUBC58 belonged to E2 subfamily XVIII of maize,and they all had UBC domain whose sequences had high similarity except gene ZmUBC17.Genes ZmUBC18,ZmUBC48,ZmUBC57,ZmUBC58 expressed in root and leaf,and almost had the same expression patterns.The expression reached the peak at 6 h when dealing wit

关 键 词：玉米 泛素结合酶(E2) 生物信息学 系统发育进化树 基因表达 

分 类 号：S513.035.3[农业科学—作物学]