培养液匀浆人参发根抗氧化、抗黑色素瘤作用  

Antioxidant and Antimelanoma Capability of Ginseng Hairy Root Culture

作　　者：王璐[1] 郜玉钢[1] 郭阳 刘杨 陈思[1] 臧埔 何忠梅[1] 赵岩[1] 张连学[1] WANG Lu;GAO Yugang;GUO Yang;LIU Yang;CHEN Si;ZANG Pu;HE Zhongmei;ZHAO Yan;ZHANG Lianxue(College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品科学》2018年第17期152-158,共7页Food Science

基　　金："十三五"国家重点研发计划重点专项(2016YFC0500300);吉林省科技厅科技发展计划项目(20160307005YY;20170204017YY);公益性行业(农业)科研专项(201303111);国家级大学生创新创业项目(201610193027)

摘　　要：以培养液匀浆人参发根为实验材料,选用Cu^(2+)螯合能力、多酚氧化酶活力、总抗氧化能力3种方法对其抗氧化能力进行测定;采用流式细胞术对黑色素瘤细胞A375凋亡、细胞周期进行测定。结果表明:随着质量浓度的增大,培养液匀浆人参发根的Cu^(2+)螯合率逐渐提高,在100 mg/m L时与VC差异不显著(P>0.05);40、60、80、100 mg/m L时培养液匀浆人参发根与VC的多酚氧化酶活力差异不显著(P>0.05);培养液匀浆人参发根的总抗氧化能力具有质量浓度依赖性,在100 mg/m L时达到0.877 4 mmol FeSO_4/g。与对照组相比培养液匀浆人参发根组细胞凋亡显著(P<0.05);S、G2/M期比例升高,G0/G1期比例下降。结论:人参发根具有抗氧化、抗黑色素瘤作用。The antioxidant capacity of Panax ginseng hairy root culture homogenate was measured by Cu2+chelating ability,polyphenol oxidase activity and total antioxidant capacity assays,and its antimelanoma activity was evaluated by determining the cell apoptosis and cycle in melanoma cells A375 using flow cytometry.The results showed that the ginseng hairy root culture had Cu2+chelating ability in a concentration-dependent manner,which was not significantly different from that of VC at a concentration of 100 mg/mL(P>0.05).No significant difference in polyphenol oxidase activity was found between the presence of the homogenate and VC at 40,60,80 and 100 mg/mL(P>0.05).The total antioxidant capacity of the homogenate was concentration dependent,0.877 4 mmol FeSO4/g at 100 mg/mL.Compared with the control group,the ginseng hairy root culture significantly increased the cell apoptosis rate(P<0.05),improved the percentage of cells in the S and G2/M phases and decreased the percentage of cells in the G0/G1 phase.These findings led to the conclusion that Panax ginseng hairy root has antioxidant and antimelanoma effects.

关 键 词：人参发根 抗氧化 黑色素瘤 细胞凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]