色氨酸合成酶基因工程菌合成S-甲基-L-半胱氨酸  

Enzymatic Synthesis of S-methyl-L-cysteine Catalyzed by Tryptophan Synthase Genetically Engineered Microorganism

作　　者：徐礼生 高贵珍[1] 曹稳根 赵亮 张兴桃 焦庆才 鲍妮娜[1] 宋曼 XU Lisheng;GAO Guizhen;CAO Wengen;ZHAO Liang;ZHANG Xingtao;JIAO Qingcai;BAO Nina;SONG Man(Department of Life and Food Science,Suzhou University,Suzhou 234000,China;State Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,Nanjing 210093,China)

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000 [2]南京大学医药生物技术国家重点实验室,江苏南京210093

出　　处：《食品与生物技术学报》2018年第6期655-660,共6页Journal of Food Science and Biotechnology

基　　金：安徽省高校自然科学研究重点项目(KJ2017A440);安徽省质量工程项目(2015zjjh034;2015ckjh108;2016jyxm1038);宿州学院学术带头人项目(2018XJXS02);宿州市科技计划项目(SZ2017GG18)

摘　　要：通过密码子优化设计构建色氨酸合成酶基因工程菌,单因素以及响应面法研究色氨酸合成酶基因工程菌制备S-甲基-L-半胱氨酸。结果表明,温度39℃、pH 8.5、L-丝氨酸浓度360mmol/L,反应平衡时间20 h,S-甲基-L-半胱氨酸的质量浓度为40.49 g/L。Tryptophan synthase genetically engineered microorganism was constructed by codon optimization in Escherichia coli.Preparation of S-methyl-L-cysteine from L-serine and methanthiol catalyzed by tryptophan synthase genetically engineered microorganism was investigated by response surface methodology and single-factor experiments.The optimal temperature and pH value were 39℃and 8.5 respectively.The optimal substrate concentration of L-serine was 360 mmol/L.Under the optimal conditions of tryptophan synthase genetically engineered microorganism,the content of S-methyl-L-cysteine was 40.49 g/L after 20 h.

关 键 词：色氨酸合成酶 S-甲基-L-半胱氨酸 L-丝氨酸 甲硫醇 

分 类 号：Q814.1[生物学—生物工程]